Department for Functional Materials in Medicine and Dentistry, Dental School, University of Würzburg, Würzburg, Germany.
J Biomed Mater Res A. 2012 Sep;100(9):2392-400. doi: 10.1002/jbm.a.34184. Epub 2012 Apr 24.
Bone substitute materials such as calcium phosphate cements (CPC) are frequently used as growth factor carriers for the stimulation of osteoblast-formation around an implant. However, biological modification based on delicate protein factors like extracellular matrix proteins or growth factors is subject to a number of shortcomings like the need for storage below room temperature and cost of production. The aim of this study was to investigate ionic modification as an alternative bioinorganic route for implant modification. Although it is known that Cu(II) plays a role in angiogenesis and bone formation, not all involved processes are well understood yet. In this study the in vitro effect of Cu(II) on growth and activity of osteoblastic cells seeded on brushite (CaHPO(4) · 2 H(2) O) scaffolds as well as on glass discs was investigated. The results show that Cu(II) enhances cell activity and proliferation of osteoblastic cells on CPC and furthermore affects the expression of several bone specific proteins such as bone sialo protein or osteocalcin. Therefore, the modification of CPC with Cu(II) may offer a promising alternative to protein based modification to stimulate cellular activity for an improved bone healing.
骨替代材料如磷酸钙骨水泥（CPC）经常被用作生长因子载体，以刺激植入物周围成骨细胞的形成。然而，基于细胞外基质蛋白或生长因子等精细蛋白质因子的生物修饰存在许多缺点，例如需要在室温以下储存和生产成本高。本研究旨在探讨离子修饰作为植入物修饰的替代生物无机途径。尽管已知 Cu(II) 在血管生成和骨形成中起作用，但并非所有涉及的过程都被很好地理解。在这项研究中，研究了 Cu(II) 对在 brushite（CaHPO4·2H2O）支架上以及玻璃片上接种的成骨细胞的生长和活性的体外影响。结果表明，Cu(II) 增强了 CPC 上成骨细胞的活性和增殖，并且还影响了几种骨特异性蛋白如骨唾液蛋白或骨钙素的表达。因此，用 Cu(II) 修饰 CPC 可能为刺激细胞活性以改善骨愈合提供了一种有前途的替代基于蛋白质的修饰方法。
