结合脱氧核糖核酸的流感嗜血杆菌周质蛋白：特性及在遗传转化中的可能作用
Haemophilus influenzae periplasmic protein which binds deoxyribonucleic acid: properties and possible participation in genetic transformation.
作者信息
Sutrina S L, Scocca J J
出版信息
J Bacteriol. 1979 Sep;139(3):1021-7. doi: 10.1128/jb.139.3.1021-1027.1979.
Abstract
A protein which binds to either single-stranded or double-stranded deoxyribonucleic acid (DNA) but not to ribonucleic acid has been isolated by osmotic shock treatment of growing cells. This periplasmic protein differs from the principal intracellular binding protein in its greater thermolability and by the absence of salt-induced cooperativity in its interaction with single-stranded DNA. Certain mutant strains of Haemophilus influenzae defective in the DNA suptake steps of genetic transformation were found to be deficient in periplasmic DNA-binding protein, suggesting that this protein participates in the uptake of DNA in transformation.
摘要
通过对生长中的细胞进行渗透休克处理，已分离出一种能与单链或双链脱氧核糖核酸（DNA）结合但不与核糖核酸结合的蛋白质。这种周质蛋白与主要的细胞内结合蛋白不同，其热稳定性更高，并且在与单链DNA相互作用时不存在盐诱导的协同性。发现某些在遗传转化的DNA摄取步骤中存在缺陷的流感嗜血杆菌突变株缺乏周质DNA结合蛋白，这表明该蛋白参与了转化过程中DNA的摄取。
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