Fukuoka Tokuma, Yanagihara Takashi, Ito Seya, Imura Tomohiro, Morita Tomotake, Sakai Hideki, Abe Masahiko, Kitamoto Dai
Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology-AIST, Tsukuba, Ibaraki 305-8565, Japan.
J Oleo Sci. 2012;61(5):285-9. doi: 10.5650/jos.61.285.
Mannosylerythritol lipids (MELs) are secreted by yeasts and are promising glycolipid biosurfactants. In our study on the non-aqueous phase behaviors of MEL homologues, we found that MEL-D (4-O-[2',3'-di-O-alka(e)noyl-β-D-mannopyranosyl]-(2R,3S)-erythritol) forms aggregates in decane. The microscopic observation and the X-ray scattering measurement of these aggregates revealed that they are reverse vesicles that consist of bilayers whose hydrophilic domains are located in the interior of the bilayers. In addition, MEL-D formed reverse vesicles without co-surfactants and co-solvents in various oily solutions, such as n-alkanes, cyclohexane, squalane, squalene, and silicone oils at a concentration below 10 mM. This is the first report on the reverse vesicle formation from biosurfactants.
甘露糖赤藓糖醇脂（MELs）由酵母分泌，是很有前景的糖脂类生物表面活性剂。在我们对MEL同系物非水相行为的研究中，我们发现MEL-D（4-O-[2',3'-二-O-链烷（烯）酰基-β-D-甘露吡喃糖基]-(2R,3S)-赤藓糖醇）在癸烷中形成聚集体。对这些聚集体的显微镜观察和X射线散射测量表明，它们是反向囊泡，由双层组成，其亲水区位于双层内部。此外，MEL-D在各种油溶液中，如正构烷烃、环己烷、角鲨烷、角鲨烯和硅油中，在浓度低于10 mM时，无需助表面活性剂和助溶剂就能形成反向囊泡。这是关于生物表面活性剂形成反向囊泡的首次报道。
