Brackett J, Durley B, Janczak R, Kazlauskas V, Kmiec J, Norlie J, Rosencranz R, Schultz S, Spring T, Theusch W
Abbott Laboratories, Diagnostics Division, Abbott Park, IL 60064.
Clin Chem. 1990 Dec;36(12):2126-30.
We describe a novel ion-selective electrode (ISE) system that can be used in the Abbott Vision analyzer. A reusable sensor pack and a disposable test pack have been miniaturized to approximately the size and weight of a conventional Vision test pack, thus eliminating the need for a separate ISE module. The sensor pack contains a fluid path, battery-powered electronics, and screw-in electrodes. The test pack contains separate chambers and fluid channels for a blood specimen and two aqueous calibrators. During a run, plasma is separated from blood cells and is moved sequentially, along with the two calibrators, into and out of the sensor pack by centrifugal force. Each run includes checks for electromechanical integrity, fluid contamination, calibration slope and intercept, and specimen hemolysis. The system operates with CVs of less than 2% and gives results that correlate well with those by flame photometry and direct ISE methods. Either skin-puncture or venipuncture whole blood can be assayed, as well as serum or plasma.
我们描述了一种可用于雅培视觉分析仪的新型离子选择性电极（ISE）系统。一个可重复使用的传感器包和一个一次性测试包已被小型化至大约传统视觉测试包的尺寸和重量，从而不再需要单独的ISE模块。传感器包包含一个流体路径、电池供电的电子设备和旋入式电极。测试包包含用于血液标本和两种水性校准液的独立腔室和流体通道。在运行过程中，血浆与血细胞分离，并通过离心力与两种校准液一起依次进出传感器包。每次运行包括检查机电完整性、流体污染、校准斜率和截距以及标本溶血情况。该系统的变异系数小于2%，其结果与火焰光度法和直接ISE法的结果相关性良好。可以检测皮肤穿刺或静脉穿刺全血，以及血清或血浆。
