Wang Ye, Zhao Cuizhu, Zhao Boyu, Duan Xinran, Hao Peng, Liang Xiaojun, Yang Lianyu, Gao Yunhang
College of Veterinary Medicine, Jilin Agricultural University, Changchun 130118, China.
Institute of Animal Science, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750002, China.
Microorganisms. 2024 Dec 16;12(12):2597. doi: 10.3390/microorganisms12122597.
Tylosin (TYL) pollution has aroused widespread concern, and its existence poses a serious threat to the environment and human health. Microbial degradation of antibiotics is considered to be an effective strategy to reduce the environmental impact of antibiotics, but its degradation mechanism is still unclear. In this study, transcriptome analysis was combined to explore the response mechanism of strain TYL-A1 under TYL stress. The results showed that the strain showed a significant antioxidant response under TYL stress to cope with TYL-induced cell damage. TYL also increased the level of intracellular reactive oxygen species (ROS), damaged the integrity of the cell membrane, and inhibited the growth of strain TYL-A1. Transcriptome sequencing showed that under TYL exposure conditions, 1650 DEGs in strain TYL-A1 showed expression changes, of which 806 genes were significantly up-regulated and 844 genes were significantly down-regulated. Differentially expressed DEGs were significantly enriched in pathways related to metabolism, biosynthesis, and stress response, and tricarboxylic acid cycle, oxidative phosphorylation, and carbon metabolism genes were significantly up-regulated. In conclusion, this study provides novel insights regarding the degradation of TYL by TYL-A1.
泰乐菌素（TYL）污染已引起广泛关注，其存在对环境和人类健康构成严重威胁。抗生素的微生物降解被认为是减少抗生素对环境影响的有效策略，但其降解机制仍不清楚。在本研究中，结合转录组分析来探究菌株TYL-A1在TYL胁迫下的响应机制。结果表明，该菌株在TYL胁迫下表现出显著的抗氧化反应以应对TYL诱导的细胞损伤。TYL还提高了细胞内活性氧（ROS）水平，破坏了细胞膜的完整性，并抑制了菌株TYL-A1的生长。转录组测序显示，在TYL暴露条件下，菌株TYL-A1中有1650个差异表达基因（DEG）表现出表达变化，其中806个基因显著上调，844个基因显著下调。差异表达的DEG在与代谢、生物合成和应激反应相关的途径中显著富集，三羧酸循环、氧化磷酸化和碳代谢基因显著上调。总之，本研究为TYL-A1降解TYL提供了新的见解。
