Dental Clinical Center, Department of Oral Pathology and Periodontology, Faculty of Stomatology, University Ss Cyril and Methodius, Skopje, Republic of Macedonia.
Hum Immunol. 2012 Jul;73(7):753-8. doi: 10.1016/j.humimm.2012.04.009. Epub 2012 Apr 23.
Genetic polymorphisms in the interleukin 10 (IL10) gene have been reported to influence the host response to microbial challenge by altering levels of cytokine expression. We analyzed nucleotide polymorphisms in the promoter region of the IL10 gene and its relation with periodontal disease in a Macedonian population. The study population consisted of 111 unrelated subjects with chronic periodontitis and 299 healthy controls. DNA was isolated and IL10 genotyping performed by PCR-SSP (Heidelberg kit) for the alleles and genotypes of IL10 -1082, IL10 -819 and IL10 -592. Frequencies of IL10 haplotypes and the haplotype zygotes were also examined. Comparisons between groups were tested using the Pearson's p-value. After Bonferroni adjustment, significant associations were detected between subjects with chronic periodontitis and IL10 genotypes (IL10 -1082/A:G was negative or protective and IL10 -1082/G:G was positive or susceptible). Cytokine polymorphism on the IL10 gene appears to be associated with susceptibility to chronic periodontitis in Macedonians.
白细胞介素 10（IL10）基因中的遗传多态性已被报道通过改变细胞因子表达水平来影响宿主对微生物挑战的反应。我们分析了马其顿人群中白细胞介素 10 基因启动子区域的核苷酸多态性及其与牙周病的关系。研究人群包括 111 名慢性牙周炎患者和 299 名健康对照者。提取 DNA 并通过 PCR-SSP（海德堡试剂盒）对白细胞介素 10 -1082、IL10 -819 和 IL10 -592 的等位基因和基因型进行基因分型。还检查了白细胞介素 10 单倍型和单倍型合子的频率。使用 Pearson p 值检验组间比较。经过 Bonferroni 调整后，在慢性牙周炎患者和白细胞介素 10 基因型之间发现了显著的关联（IL10 -1082/A:G 为阴性或保护，IL10 -1082/G:G 为阳性或易感）。白细胞介素 10 基因上的细胞因子多态性似乎与马其顿人慢性牙周炎的易感性有关。
