Tianjin Life Science Research Center and Basic Medical School, Tianjin Medical University, Tianjin, China.
IUBMB Life. 2012 Jul;64(7):628-35. doi: 10.1002/iub.1030. Epub 2012 Apr 27.
MicroRNAs are a class of small endogenous non-coding RNAs that function as post-transcriptional regulators. In our previous study, we found that miR-181b was significantly downregulated in human gastric adenocarcinoma tissue samples compared to the adjacent normal gastric tissues. In this study, we confirm the down-regulation of miR-181b in human gastric cancer cell lines versus the gastric epithelial cells. Overexpression of miR-181b suppressed the proliferation and colony formation rate of gastric cancer cells. miR-181b downregulated the expression of cAMP responsive element binding protein 1 (CREB1) by binding its 3' untranslated region. Overexpression of CREB1 counteracted the suppression of growth in gastric cancer cells caused by ectopic expression of miR-181b. These results indicate that miR-181b may function as a tumor suppressor in gastric adenocarcinoma cells through negative regulation of CREB1.
MicroRNAs 是一类小的内源性非编码 RNA，作为转录后调控因子发挥作用。在我们之前的研究中，我们发现 miR-181b 在人胃腺癌组织样本中与相邻正常胃组织相比显著下调。在这项研究中，我们证实了 miR-181b 在人胃癌细胞系与胃上皮细胞中的下调。miR-181b 的过表达抑制了胃癌细胞的增殖和集落形成率。miR-181b 通过结合其 3'非翻译区下调 cAMP 反应元件结合蛋白 1 (CREB1) 的表达。过表达 CREB1 逆转了外源性表达 miR-181b 对胃癌细胞生长的抑制作用。这些结果表明，miR-181b 可能通过负调控 CREB1 在胃腺癌细胞中发挥肿瘤抑制作用。
