Department of Anatomy, Histology and Physiology, University of Veterinary Medicine and Pharmacy, Kosice, Slovak Republic.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2012;47(9):1319-28. doi: 10.1080/10934529.2012.672140.
The present study was conducted to investigate the impact of carbamate insecticide - bendiocarb on the testicular structure of adult rabbits. Bendiocarb was perorally administered daily for 10 and 30 days, at a dose 5 mg/kg of body weight. After the histological sampling the tissues were investigated and compared with control. After the bendiocarb administration the absolute and relative testicular weight decreased significantly (P < 0.001) in both time periods. The testicular parenchyma showed structural changes such the sloughing of developing sex cells, occurrence of vacuoles within Sertoli cells and inside various spermatogenic cells. The interstitial Leydig cells were smaller than their control counterpart and possessed shrivelled nuclei and strongly vacuolar dark cytoplasm. The rate of changes was directly proportional on duration of the experiment. The ultrastructural examination proved presence of various cellular defects across the germinal epithelium as well as within the interstitial Leydig cells in both experimental periods. Morphometric analysis manifested decrease in diameters of seminiferous tubules, increase of the diameters of the tubular lumina due to reduction of height of the seminiferous epithelium. Results of this study show distinct negative effects of bendiocarb on structure of rabbit testes.
本研究旨在探讨氨基甲酸酯类杀虫剂丁硫克百威对成年兔睾丸结构的影响。丁硫克百威经口每日给药，剂量为 5mg/kg 体重，连续给药 10 天和 30 天。组织学取样后，与对照组进行比较。在丁硫克百威给药后，两个时间段的睾丸绝对重量和相对重量均显著下降（P<0.001）。睾丸实质显示出结构变化，如发育中的性细胞脱落、支持细胞内和各种精子发生细胞内出现空泡。间质莱迪希细胞比对照小，细胞核皱缩，细胞质暗有空泡。变化的速度与实验持续时间成正比。超微结构检查证明，在两个实验期内，生殖上皮和间质莱迪希细胞内均存在各种细胞缺陷。形态计量分析显示，由于生精上皮高度降低，曲细精管直径减小，管腔直径增大。本研究结果表明，丁硫克百威对兔睾丸结构有明显的不良影响。
