Department of Engineering Chemistry, K.L.E. Society's K.L.E. Institute of Technology, Hubli 580 030, India.
Bioelectrochemistry. 2012 Dec;88:76-83. doi: 10.1016/j.bioelechem.2012.06.004. Epub 2012 Jun 28.
Electrochemical oxidation of acyclovir at fullerene-C(60)-modified glassy carbon electrode has been investigated using cyclic and differential pulse voltammetry. In pH 7.4 phosphate buffer, acyclovir showed an irreversible oxidation peak at about 0.96V. The cyclic voltammetric results showed that fullerene-C(60)-modified glassy carbon electrode can remarkably enhance electrocatalytic activity towards the oxidation of acyclovir. The electrocatalytic behavior was further exploited as a sensitive detection scheme for the acyclovir determination by differential pulse voltammetry. Effects of anodic peak potential (E(p)/V), anodic peak current (I(p)/μA) and heterogeneous rate constant (k(0)) have been discussed. Under optimized conditions, the concentration range and detection limit were 9.0×10(-8) to 6.0×10(-6)M and 1.48×10(-8)M, respectively. The proposed method was applied to acyclovir determination in pharmaceutical samples and human biological fluids such as urine and blood plasma as a real sample. This method can also be employed in quality control and routine determination of drugs in pharmaceutical formulations.
在 pH 7.4 的磷酸盐缓冲液中，阿昔洛韦在大约 0.96V 处显示出一个不可逆的氧化峰。循环伏安法的结果表明，富勒烯-C(60)-修饰的玻碳电极可以显著增强阿昔洛韦氧化的电催化活性。电催化行为进一步被开发为一种通过差分脉冲伏安法测定阿昔洛韦的灵敏检测方案。讨论了阳极峰电位 (E(p)/V)、阳极峰电流 (I(p)/μA) 和异质速率常数 (k(0)) 的影响。在优化条件下，浓度范围和检测限分别为 9.0×10(-8) 至 6.0×10(-6)M 和 1.48×10(-8)M。该方法已应用于药物样品和人体生物流体（如尿液和血浆）中的阿昔洛韦测定，作为实际样品。该方法还可用于药物制剂中药物的质量控制和常规测定。
