Department of Obstetrics and Gynaecology, The University of Auckland, New Zealand.
J Reprod Immunol. 2012 Jun;94(2):151-4. doi: 10.1016/j.jri.2012.03.487. Epub 2012 Apr 27.
Antiphospholipid antibodies, a maternal risk factor for preeclampsia, increase shedding of necrotic trophoblast debris from the placenta, leading to endothelial dysfunction. Using Affymetrix HGU133 Plus 2 microarrays we found changes in the transcriptome of placental explants treated with antiphospholipid antibodies, including mRNAs BCL2L1, MCL1, PDCD2L, FASLG, SEMA6A, PRKCE and TRAIL that are involved in the regulation of apoptosis. Quantitative real-time RT-PCR and immunohistochemistry confirmed a reduction in TRAIL expression in response to antiphospholipid antibodies. These results may help to understand how antiphospholipid antibodies affect trophoblast cell death and how the antibodies could contribute to the pathogenesis of preeclampsia.
抗磷脂抗体是子痫前期的一种母体危险因素，它会增加胎盘坏死滋养层碎片的脱落，导致内皮功能障碍。我们使用 Affymetrix HGU133 Plus 2 微阵列发现，抗磷脂抗体处理的胎盘外植体的转录组发生了变化，包括 BCL2L1、MCL1、PDCD2L、FASLG、SEMA6A、PRKCE 和 TRAIL 等 mRNA，它们参与凋亡的调节。实时定量 RT-PCR 和免疫组织化学证实，抗磷脂抗体反应导致 TRAIL 表达减少。这些结果可能有助于理解抗磷脂抗体如何影响滋养层细胞死亡，以及抗体如何导致子痫前期的发病机制。
