使用导管败血症的体内实验模型评估一种新的集线器设计和半定量导管培养方法。
Assessment of a new hub design and the semiquantitative catheter culture method using an in vivo experimental model of catheter sepsis.
作者信息
Segura M, Alía C, Valverde J, Franch G, Torres Rodríguez J M, Sitges-Serra A
机构信息
Department of Surgery, Hospital Universitari Nostra Senyora del Mar, Barcelona, Spain.
出版信息
J Clin Microbiol. 1990 Nov;28(11):2551-4. doi: 10.1128/jcm.28.11.2551-2554.1990.
An in vivo model of hub-related catheter sepsis in rabbits is reported. The model was used to investigate the protection offered by a new hub design against external contamination by Pseudomonas aeruginosa or Staphylococcus epidermidis and to reassess the diagnostic value of the semiquantitative culture method in bacteremia of endoluminal origin. Contamination of conventional Luer-Lock connectors was followed by clinical sepsis, positive catheter segment cultures, or both, whereas contamination of the new hub was followed by complete protection. Clinical and bacteriological discrepancies observed between contamination with P. aeruginosa and S. epidermidis suggest that the virulence of microorganisms may account for differences in the natural history of hub-originated catheter sepsis. The semiquantitative extraluminal method for catheter culture yielded less than 15 CFU in three animals with proven bacteremia and should not be used as the "gold standard" to define catheter-related bacteremia.
报道了一种兔体内与接头相关的导管败血症模型。该模型用于研究一种新型接头设计对铜绿假单胞菌或表皮葡萄球菌外部污染的防护作用，并重新评估半定量培养法在腔内源性菌血症中的诊断价值。传统鲁尔锁接头受到污染后会出现临床败血症、导管段培养阳性或两者皆有，而新型接头受到污染后则有完全的防护作用。铜绿假单胞菌和表皮葡萄球菌污染之间观察到的临床和细菌学差异表明，微生物的毒力可能是接头源性导管败血症自然病程差异的原因。导管培养的半定量腔外方法在三只已证实菌血症的动物中产生的菌落形成单位少于15个，不应作为定义导管相关菌血症的“金标准”。
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