Cooper G L, Schiller A L, Hopkins C C
Infection Control Unit, Massachusetts General Hospital, Boston 02114.
J Clin Microbiol. 1988 Jan;26(1):8-12. doi: 10.1128/jcm.26.1.8-12.1988.
An animal model of vascular-catheter-associated dermal tunnel infections was developed to study the pathogenesis of such infections. Bacteria inoculated onto entry sites of catheters into skin could be identified by culture and Gram stain on the tips of plastic catheters (4 cm from the entry site) within 1 h of inoculation, whether the animal was inoculated at the time of insertion of the catheter or 1 week afterwards. Histological examination of dermal tunnels revealed that the introduction of bacteria preceded the development of tissue inflammation. Bacteria on entry sites of percutaneous catheters moved rapidly from the entry site into the dermal tunnel along the external catheter surface, perhaps suspended in a fluid phase and propelled by capillary action.
为了研究血管导管相关皮肤隧道感染的发病机制，建立了一种动物模型。接种在导管皮肤入口处的细菌，无论动物是在导管插入时还是插入后1周接种，均可在接种后1小时内通过塑料导管尖端（距入口处4厘米）的培养和革兰氏染色进行鉴定。皮肤隧道的组织学检查显示，细菌的引入先于组织炎症的发展。经皮导管入口处的细菌沿着导管外表面从入口迅速进入皮肤隧道，可能悬浮在液相中并由毛细作用推动。
