Departament de Biología Marina i Oceanografía, Institut de Ciències del Mar, CSIC, Passeig Marítim de la Barceloneta 37-49, 08003 Barcelona, Spain.
Mar Biotechnol (NY). 2013 Feb;15(1):37-47. doi: 10.1007/s10126-012-9450-7. Epub 2012 Apr 29.
The principal fatty acids from the lipid profiles of two autochthonous dinoflagellates (Alexandrium minutum and Karlodinium veneficum) and one raphidophyte (Heterosigma akashiwo) maintained in bubble column photobioreactors under outdoor culture conditions are described for the first time. The biomass production, lipid content and lipid productivity of these three species were determined and the results compared to those obtained when the strains were cultured indoors. Under the latter condition, the biotic values did not significantly differ among species, whereas under outdoor conditions, differences in both duplication time and fatty acids content were observed. Specifically, A. minutum had higher biomass productivity (0.35 g·L⁻¹ day⁻¹), lipid productivity (80.7 mg lipid·L⁻¹ day⁻¹) and lipid concentration (252 mg lipid·L⁻¹) at harvest time (stationary phase) in outdoor conditions. In all three strains, the growth rate and physiological response to the light and temperature fluctuations of outdoor conditions greatly impacted the production parameters. Nonetheless, the species could be successfully grown in an outdoor photobioreactor and were of sufficient robustness to enable the establishment of long-term cultures yielding consistent biomass and lipid production.
首次描述了在户外培养条件下，在鼓泡式光生物反应器中培养的两种土著腰鞭毛藻（亚历山大藻和卡氏根管藻）和一种甲藻（膝沟藻属）的脂质图谱中的主要脂肪酸。测定了这三种生物的生物量产量、脂质含量和脂质生产力，并将结果与室内培养时的结果进行了比较。在后者的条件下，生物量没有显著的种间差异，而在户外条件下，复制时间和脂肪酸含量都存在差异。具体而言，在户外条件下，亚历山大藻在收获时间（静止期）具有更高的生物量生产力（0.35 g·L⁻¹ day⁻¹）、脂质生产力（80.7 mg 脂质·L⁻¹ day⁻¹）和脂质浓度（252 mg 脂质·L⁻¹）。在所有三种菌株中，生长速率和对户外条件下光照和温度波动的生理响应极大地影响了生产参数。尽管如此，这些物种可以在户外光生物反应器中成功生长，并且具有足够的稳健性，可以建立长期的培养，从而实现稳定的生物量和脂质生产。
