Oka K, Mori N, Haimoto H, Kato K
Section of Clinical Laboratory, Hospital, National Institute of Radiological Sciences, Chiba, Japan.
Lab Invest. 1990 Dec;63(6):792-7.
Frozen biopsy specimens taken from 30 cases with T cell tumors (8 with T cell acute lymphoblastic leukemia, 8 with T cell lymphoblastic lymphoma, and 14 with peripheral T cell lymphomas), and from 12 with Hodgkin's disease, were investigated using a direct immunohistochemical method to detect alpha-, beta- and gamma-enolases. Normal thymus and lymph node specimens with reactive lymphadenitis were also investigated. Subcortical thymocytes and the majority of deep cortical thymocytes showed reactivity of alpha-/beta-/gamma- approximately +/- -enolases, and medullary thymocytes and small lymphocytes in T zone areas of lymph node showed reactivity of alpha-/beta+/gamma- approximately +/- -enolases. Seven of the 8 cases with T cell acute lymphoblastic leukemia showed reactivity of alpha-/beta-/gamma(-)-enolases or alpha+/beta-/gamma(-)-enolases in leukemic lymphocytes, 7 of the 8 cases with T cell lymphoblastic lymphoma showed reactivity of beta(+)-enolase, and all 14 cases with peripheral T-cell lymphomas showed reactivity of alpha-/beta- approximately +/gamma(+)-enolases in lymphoma cells. All the 12 cases with Hodgkin's disease showed reactivity of alpha-/beta+/gamma(+)-enolases in Reed-Sternberg and Hodgkin's cells. These results indicate the following: (a) The neoplastic cells of T cell acute lymphoblastic leukemia, T cell lymphoblastic lymphoma and peripheral T cell lymphomas present different expressions in each of these three categories. This may imply a difference of maturation and differentiation or activation among neoplastic T lymphocytes. (b) T lymphocytes may switch from alpha- to beta-enolase and from alpha- to gamma-enolase in the course of differentiation and activation. (c) It is worth noting that the Reed-Sternberg and Hodgkin's cells of Hodgkin's disease present an identical expression of enolases.
采用直接免疫组织化学方法，对取自30例T细胞肿瘤（8例T细胞急性淋巴细胞白血病、8例T细胞淋巴母细胞淋巴瘤和14例外周T细胞淋巴瘤）以及12例霍奇金病患者的冷冻活检标本进行检测，以检测α-、β-和γ-烯醇化酶。同时也对正常胸腺和伴有反应性淋巴结炎的淋巴结标本进行了研究。皮质下胸腺细胞和大多数深层皮质胸腺细胞显示α-/β-/γ-烯醇化酶呈近似±的反应性，而髓质胸腺细胞以及淋巴结T区的小淋巴细胞显示α-/β+/γ-烯醇化酶呈近似±的反应性。8例T细胞急性淋巴细胞白血病患者中有7例白血病淋巴细胞显示α-/β-/γ(-)-烯醇化酶或α+/β-/γ(-)-烯醇化酶的反应性，8例T细胞淋巴母细胞淋巴瘤患者中有7例显示β(+)-烯醇化酶的反应性，14例外周T细胞淋巴瘤患者的淋巴瘤细胞均显示α-/β-近似+/γ(+)-烯醇化酶的反应性。12例霍奇金病患者的里德-斯腾伯格细胞和霍奇金细胞均显示α-/β+/γ(+)-烯醇化酶的反应性。这些结果表明：(a) T细胞急性淋巴细胞白血病、T细胞淋巴母细胞淋巴瘤和外周T细胞淋巴瘤的肿瘤细胞在这三类中各自呈现不同的表达。这可能意味着肿瘤性T淋巴细胞在成熟、分化或激活方面存在差异。(b) T淋巴细胞在分化和激活过程中可能从α-烯醇化酶转换为β-烯醇化酶，以及从α-烯醇化酶转换为γ-烯醇化酶。(c) 值得注意的是，霍奇金病的里德-斯腾伯格细胞和霍奇金细胞呈现相同的烯醇化酶表达。
