Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan.
Phytochemistry. 2012 Jul;79:141-6. doi: 10.1016/j.phytochem.2012.03.007. Epub 2012 May 1.
Significant attention has been devoted to studying hairy root cultures as a promising strategy for production of various valuable secondary metabolites. These offer many advantages, such as high growth rate, genetic stability and being hormone-free. In this study, a detailed phytochemical investigation of the secondary metabolites of Coleus forskohlii hairy root cultures was undertaken and which resulted in the isolation of 22 compounds, including four forskolin derivatives and a monoterpene. Their structures were elucidated by extensive spectroscopic analyses. These compounds could be classified into four groups viz.: labdane-type diterpenes, monoterpenes, triterpenes and phenylpropanoid dimers. Apart from one compound, all labdane type diterpenes are oxygenated at C-11 as in forskolin and a scheme showing their biosynthetic relationships is proposed.
人们对发根培养物进行了大量研究，将其作为生产各种有价值的次生代谢产物的有前途的策略。这些培养物具有许多优点，例如生长速度快、遗传稳定性和无激素。在这项研究中，对毛喉鞘蕊花发根培养物的次生代谢产物进行了详细的植物化学研究，分离得到了 22 种化合物，包括 4 种毛喉鞘蕊花苷衍生物和 1 种单萜。通过广泛的光谱分析确定了它们的结构。这些化合物可以分为四类：贝壳杉烷型二萜、单萜、三萜和苯丙素二聚体。除了一种化合物外，所有贝壳杉烷型二萜都在 C-11 位上被氧化，类似于毛喉鞘蕊花苷，提出了一个表明它们生物合成关系的示意图。
