Zhuang Pei, Shen Yi, Chiou George C Y
Institute of Ocular Pharmacology, College of Medicine, Texas A&M Health Science Center, College Station, TX 77843, USA.
Int J Ophthalmol. 2010;3(2):99-103. doi: 10.3980/j.issn.2222-3959.2010.02.02. Epub 2010 Jun 18.
To investigate the effect of flavone on oxidation-induced injury in retinal pigment epithelium cells.
In in vivo studies, NaIO(3)-induced RPE degeneration in rat eyes was treated with 0.5% flavone eye drops 3 times a day for 1 week before and 4 weeks after NaIO(3) injection. At the end of 2 and 4 weeks, all rats were measured c-wave by electroretinogram (ERG). In in vitro studies, ARPE-19 cells were treated with hypoxia, H(2)O(2), NaN(3) and t-BHP to induce cell damages. MTT assay was used to measure the viable cells.
The ERG c-wave results showed that flavone reversed NaIO(3)-induced injury at the end of 4 weeks. In vitro results showed flavone reversed the various oxidants-induced injuries in RPE cells.
Flavone could prevent the RPE from oxidation-induced injury both in vivo and in vitro.
研究黄酮对视网膜色素上皮细胞氧化损伤的影响。
在体内研究中，用0.5%黄酮滴眼液对大鼠眼内注射碘酸钠（NaIO₃）诱导的视网膜色素上皮（RPE）变性进行治疗，在注射NaIO₃前1周及注射后4周，每天滴眼3次。在第2周和第4周结束时，通过视网膜电图（ERG）测量所有大鼠的c波。在体外研究中，用缺氧、过氧化氢（H₂O₂）、叠氮化钠（NaN₃）和叔丁基过氧化氢（t-BHP）处理ARPE-19细胞以诱导细胞损伤。采用MTT法检测活细胞数量。
ERG的c波结果显示，在第4周结束时黄酮可逆转NaIO₃诱导的损伤。体外实验结果表明，黄酮可逆转各种氧化剂诱导的RPE细胞损伤。
黄酮在体内和体外均可预防RPE细胞的氧化损伤。
