人参皂苷-Rg3对糖尿病大鼠视网膜中血管内皮生长因子（VEGF）和肿瘤坏死因子-α（TNF-α）表达的影响。
Effect of ginsenoside-Rg3 on the expression of VEGF and TNF-α in retina with diabetic rats.
作者信息
Sun Hong-Quan, Zhou Zhan-Yu
机构信息
Department of Ophthalmology, the Affiliated Hospital of Medical College of Qingdao University, Qingdao 266003, Shandong Province, China.
出版信息
Int J Ophthalmol. 2010;3(3):220-3. doi: 10.3980/j.issn.2222-3959.2010.03.09. Epub 2010 Sep 18.
AIM
To investigate the effect of ginsenoside-Rg3 on the expression of vascular endothelial growth factor (VEGF) and tumor necrosis factor-α (TNF-α) in retina with diabetic rats and its roles in preventing neovascularization in diabetes.
METHODS
Sixty male Wistar rats were divided into 3 groups randomly: negative control group, diabetic control group and ginsenoside-Rg3 treatment group (5mg/kg, 0.2mg/mL) followed by establishing diabetic model. The expression of VEGF and TNF-α were measured after 8 weeks.
RESULTS
There were significant differences among negative control group, diabetic control group and ginsenoside-Rg3 treatment group in the expression of VEGF and TNF-α (F=129.363, 211.992; all the P<0.01). VEGF and TNF-α expression were significantly higher in diabetic control group and ginsenoside-Rg3 treatment group than that in negative control group (P<0.01), with a significant reduction in ginsenoside-Rg3 treatment group than that in diabetic control group (P<0.01).
CONCLUSION
Ginsenoside-Rg3 can down-regulate the expression of VEGF and TNF-α in retina, which may interfere in the development of diabetic retinopathy.
目的
探讨人参皂苷-Rg3对糖尿病大鼠视网膜血管内皮生长因子（VEGF）和肿瘤坏死因子-α（TNF-α）表达的影响及其在预防糖尿病新生血管形成中的作用。
方法
60只雄性Wistar大鼠随机分为3组：阴性对照组、糖尿病对照组和人参皂苷-Rg3治疗组（5mg/kg，0.2mg/mL），随后建立糖尿病模型。8周后检测VEGF和TNF-α的表达。
结果
阴性对照组、糖尿病对照组和人参皂苷-Rg3治疗组之间VEGF和TNF-α的表达有显著差异（F=129.363，211.992；均P<0.01）。糖尿病对照组和人参皂苷-Rg3治疗组中VEGF和TNF-α的表达显著高于阴性对照组（P<0.01），人参皂苷-Rg3治疗组较糖尿病对照组显著降低（P<0.01）。
结论
人参皂苷-Rg3可下调视网膜中VEGF和TNF-α的表达，这可能会干扰糖尿病视网膜病变的发展。
Zotero 插件