Yuuki T, Kanda T, Kimura Y, Kotajima N, Tamura J, Kobayashi I, Kishi S
Department of Ophthalmology, Gunma University School of Medicine, Maebashi, Japan.
J Diabetes Complications. 2001 Sep-Oct;15(5):257-9. doi: 10.1016/s1056-8727(01)00155-6.
To determine whether inflammatory cytokines are increased in proliferative diabetic retinopathy. We measured concentrations of interleukin-6, 8 (IL-6, 8) and tumor necrosis factor (TNF)-alpha by enzyme-linked immunosorbent assay (ELISA) in vitreous and serum from 47 patients with proliferative diabetic retinopathy and 21 patients with vitreous noninflammatory retinopathies. Vitreous concentration of IL-6 were 64.7+/-12.8 pg/ml in proliferative diabetic retinopathy, much greater (P<.005) than in noninflammatory retinopathy (2.8+/-4.5 pg/ml). Amounts of IL-8 in vitreous fluid also were greater in proliferative retinopathy than in noninflammatory retinopathy (34.0+/-11.5 vs. 6.1+/-2.0 pg/ml, P<.005). Concentrations of TNF-alpha in vitreous fluid were not statistically different in proliferative retinopathy from those in noninflammatory retinopathy. In sera, concentrations of IL-6 and IL-8 were not different between proliferative and noninflammatory retinopathy. However, serum TNF-alpha was much greater in proliferative retinopathy than in noninflammatory retinopathy (0.81+/-0.72 vs. 0.09+/-0.00 pg/ml, P<.001). Elevated TNF-alpha in serum then may be diagnostically useful in proliferative diabetic retinopathy. And inflammatory cytokines in vitreous may be pathogenically important in this concentration.
为了确定增殖性糖尿病视网膜病变中炎性细胞因子是否增加。我们通过酶联免疫吸附测定（ELISA）测量了47例增殖性糖尿病视网膜病变患者和21例玻璃体非炎性视网膜病变患者的玻璃体和血清中白细胞介素-6、8（IL-6、8）和肿瘤坏死因子（TNF）-α的浓度。增殖性糖尿病视网膜病变患者玻璃体中IL-6浓度为64.7±12.8 pg/ml，远高于非炎性视网膜病变患者（2.8±4.5 pg/ml，P<0.005）。增殖性视网膜病变患者玻璃体中IL-8的含量也高于非炎性视网膜病变患者（34.0±11.5 vs. 6.1±2.0 pg/ml，P<0.005）。增殖性视网膜病变患者玻璃体中TNF-α的浓度与非炎性视网膜病变患者相比无统计学差异。在血清中，增殖性和非炎性视网膜病变患者的IL-6和IL-8浓度无差异。然而，增殖性视网膜病变患者血清TNF-α远高于非炎性视网膜病变患者（0.81±0.72 vs. 0.09±0.00 pg/ml，P<0.001）。因此，血清中TNF-α升高可能对增殖性糖尿病视网膜病变具有诊断价值。玻璃体中的炎性细胞因子在这种浓度下可能具有致病重要性。
