Yang Ling-Ling, Zhou Qing-Jun, Wang Yao, Gao Yan, Wang Yi-Qiang
State Key Laboratory Cultivation Base, Shandong Provincial Key Lab of Ophthalmology, Shandong Eye Institute, Qingdao, China.
Int J Ophthalmol. 2012;5(1):32-7. doi: 10.3980/j.issn.2222-3959.2012.01.07. Epub 2012 Feb 18.
To compare the therapeutic effects of polysaccharide extract from Spirulina platensis (PSP) and extract from amnion membrane (AME) on alkali burn-induced corneal neovascularization (CorNV).
PSP and AME were extracted from dry powder of Spirulina platensis and human aminion membrane respectively. Murine CorNV was induced by applying 1N sodiumhydroxide (NaOH) solution directly on the mice corneas. PSP and AME extracts were administered topically on the corneas 4 times daily for 7 days. The therapy effects of PSP and AME extracts were evaluated daily using slit-lamp. At the end of the therapy, corneas were harvested for H&E staining, masson trichrome staining, immunohistochemical study, and semi-quantification reverse transcriptive PCR (RT-PCR) was utilized for measurement of inflammation-related molecules.
Topical application of PSP extract had significant therapeutic effects on CorNV that could be shown in various assays of the corneas. Compared with AME extract, PSP extract treatment was more effective in suppressing CorNV in terms of vessel length and levels of cluster of differentiation 31 (CD31) proteins or the angiogenesis related genes like vascular endothelial growth factor (VEGF), matrix metalloproteinase-2 (MMP2) and matrix metalloproteinase-9 (MMP9). PSP also inhibited inflammation more markedly by more effectively inhibiting mononuclear and polymorphonuclear cells infiltration into the corneal stroma and reducing levels of stromal cell-derived factor-1 (SDF1), tumor necrosis factor-alpha (TNFα) and macrophage inflammatory protein-3 (MIP3a). In additon, corneas of PSP group had a more regular and compact architecture of collagen with thinner corneal thickness than in the AME group.
Polysaccharide extract from Spirulina platensis inhibited alkali burn-induced inflammation and CorNV more effectively than AME extract at the studied doses, thus may be used for the therapy of corneal diseases involving neovascularization and inflammation.
比较钝顶螺旋藻多糖提取物（PSP）和羊膜提取物（AME）对碱烧伤诱导的角膜新生血管化（CorNV）的治疗效果。
PSP和AME分别从钝顶螺旋藻干粉和人羊膜中提取。通过将1N氢氧化钠（NaOH）溶液直接应用于小鼠角膜诱导小鼠CorNV。PSP和AME提取物每天局部应用于角膜4次，共7天。每天使用裂隙灯评估PSP和AME提取物的治疗效果。治疗结束时，收获角膜进行苏木精-伊红染色、Masson三色染色、免疫组织化学研究，并利用半定量逆转录聚合酶链反应（RT-PCR）测量炎症相关分子。
PSP提取物局部应用对CorNV具有显著治疗效果，这在角膜的各种检测中都可以显示出来。与AME提取物相比，PSP提取物治疗在抑制血管长度以及分化簇31（CD31）蛋白水平或血管内皮生长因子（VEGF）、基质金属蛋白酶-2（MMP2）和基质金属蛋白酶-9（MMP9）等血管生成相关基因方面对抑制CorNV更有效。PSP还通过更有效地抑制单核和多形核细胞浸润到角膜基质中并降低基质细胞衍生因子-1（SDF1）、肿瘤坏死因子-α（TNFα）和巨噬细胞炎性蛋白-3（MIP3a）水平，更显著地抑制炎症。此外,PSP组角膜的胶原结构比AME组更规则、更紧密，角膜厚度更薄。
在研究剂量下，钝顶螺旋藻多糖提取物比AME提取物更有效地抑制碱烧伤诱导的炎症和CorNV，因此可用于治疗涉及新生血管化和炎症的角膜疾病。
