David H. Smith Center for Vaccine Biology and Immunology, Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, NY 14642, USA.
Vaccine. 2012 Jun 29;30(31):4581-4. doi: 10.1016/j.vaccine.2012.04.059. Epub 2012 Apr 30.
Although previous studies have found minimal changes in CD4 T cell responses after vaccination of adults with trivalent inactivated influenza vaccine, daily sampling and monitoring of the proliferation marker Ki-67 have now been used to reveal that a substantial fraction of influenza-specific CD4 T cells respond to vaccination. At 4-6 days after vaccination, there is a sharp rise in the numbers of Ki-67-expressing PBMC that produce IFNγ, IL-2 and/or TNFα in vitro in response to influenza vaccine or peptide. Ki-67(+) cell numbers then decline rapidly, and 10 days after vaccination, both Ki-67(+) and overall influenza-specific cell numbers are similar to pre-vaccination levels. These results provide a tool for assessing the quality and quantity of CD4 T cell responses to different influenza vaccines, and raise the possibility that the anti-influenza T cell memory response may be qualitatively altered by vaccination, even if the overall memory cell numbers do not change significantly.
虽然之前的研究发现成人接种三价灭活流感疫苗后，CD4 T 细胞反应仅有微小变化，但现在已采用每日取样和监测增殖标志物 Ki-67 的方法，揭示出相当一部分流感特异性 CD4 T 细胞对疫苗接种有反应。在接种后 4-6 天，产生 IFNγ、IL-2 和/或 TNFα的流感疫苗或肽体外反应中，表达 Ki-67 的 PBMC 的数量急剧增加。Ki-67(+)细胞数量随后迅速下降，接种后 10 天，Ki-67(+)和整体流感特异性细胞数量与接种前水平相似。这些结果为评估不同流感疫苗的 CD4 T 细胞反应的质量和数量提供了一种工具，并提出了即使总记忆细胞数量没有显著变化，疫苗接种也可能改变抗流感 T 细胞记忆反应的质量的可能性。
