Department of Pharmacology & Toxicology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand 388001, Gujarat, India.
Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand 388001, Gujarat, India.
Environ Toxicol Pharmacol. 2012 Sep;34(2):192-199. doi: 10.1016/j.etap.2012.04.002. Epub 2012 Apr 13.
Immunotoxicity for subacute exposure to acephate (O,S-dimethyl-acetylphosphoramidothioate) was assessed in day old White Leghorn (WLH) cockerel chicks. The chicks were divided into five groups. Groups C1 and C2 served as plain control and vehicle control respectively. Chicks of groups T1, T2 and T3 were administered acephate suspended in groundnut oil at 21.3mg/kg, 28.4mg/kg and 42.6mg/kg respectively orally for 28 days. A non-significant reduction in total leukocyte count was observed. Although, anti-Newcastle Disease Virus (NDV) antibody titer, serum total protein (TP), serum globulin, serum albumin and organ:body weight ratios of immune organs were significantly suppressed. The delayed type hypersensitivity response to 2,4-dinitro-1-chlorobenzene (DNCB) was not significantly altered. Histopathologically, bursa and spleen showed mild depletion of lymphocytes. Furthermore, DNA fragmentation assay was performed and detected ladder pattern (180bp) in DNA. It was concluded that subacute acephate exposure at low concentrations may affect immune responses in avian species.
急性接触乙酰甲胺磷（O，S-二甲基乙酰基硫代磷酰胺）的免疫毒性在一日龄白来航（WLH）雏鸡中进行了评估。雏鸡被分为五组。C1 和 C2 组分别作为普通对照组和载体对照组。T1、T2 和 T3 组的雏鸡分别经口给予悬浮在花生油中的乙酰甲胺磷，剂量分别为 21.3mg/kg、28.4mg/kg 和 42.6mg/kg，连续 28 天。总白细胞计数虽略有下降，但抗新城疫病毒（NDV）抗体滴度、血清总蛋白（TP）、血清球蛋白、血清白蛋白和免疫器官/体重比值均显著降低。对 2,4-二硝基-1-氯苯（DNCB）的迟发型超敏反应没有明显改变。组织病理学检查显示，法氏囊和脾脏的淋巴细胞明显减少。此外，还进行了 DNA 片段化分析，检测到 DNA 中的梯状模式（180bp）。结论是，低浓度的乙酰甲胺磷急性接触可能会影响禽类的免疫反应。
