Adhesion Properties of Dad-13 and Mut-7 on Sprague Dawley Rat Intestine.

Adhesion capacity is considered one of the selection criteria for probiotic strains. The purpose of this study was to determine the adhesion properties of two candidate probiotics, Dad-13 and Mut-7. The evaluation included the hydrophobicity of the cell surface using microbial adhesion to hydrocarbons (MATH), autoaggregation, and the adhesion of Dad-13 and Mut-7 to the intestinal mucosa of Sprague Dawley rat, followed by genomic analysis of the two strains. Dad-13 and Mut-7 showed a high surface hydrophobicity (78.9% and 83.5%) and medium autoaggregation ability (40.9% and 57.5%, respectively). The exposure of both isolates to the surface of the rat intestine increased the total number of lactic acid bacteria on the colon compartment, from 2.9 log CFU/cm to 4.4 log CFU/cm in Dad-13 treatment and to 3.86 log CFU/cm in Mut-7 treatment. The results indicate the ability of two to attach to the surface of the rat intestine. The number of indigenous in the colon also decreased when the compartment was exposed to Dad-13 and Mut-7, from 2.9 log CFU/cm to 1 log CFU/cm. Genomic analysis revealed that both strains have genes related to adhesion properties that could play an important role in increasing the adherence of probiotics to the intestinal mucosa such as gene encoding fibronectin-binding protein, chaperonin heat shock protein 33 (Hsp33) and genes related to the capsule and cell wall biosynthesis. Based on these findings, we believe that Dad-13 and Mut-7 have adhesion properties to the intestinal mucosa in the rat intestine model system. The present research will be essential to elucidate the molecular mechanism associated with adhesion in our two probiotic strains.