Darmastuti Arum, Hasan Pratama N, Wikandari Rachma, Utami Tyas, Rahayu Endang S, Suroto Dian Anggraini
Faculty of Agricultural Technology, Universitas Gadjah Mada, Flora Street No 1 Bulaksumur, Yogyakarta 55281, Indonesia.
Center for Food and Nutrition Studies, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.
Microorganisms. 2021 Nov 11;9(11):2336. doi: 10.3390/microorganisms9112336.
Adhesion capacity is considered one of the selection criteria for probiotic strains. The purpose of this study was to determine the adhesion properties of two candidate probiotics, Dad-13 and Mut-7. The evaluation included the hydrophobicity of the cell surface using microbial adhesion to hydrocarbons (MATH), autoaggregation, and the adhesion of Dad-13 and Mut-7 to the intestinal mucosa of Sprague Dawley rat, followed by genomic analysis of the two strains. Dad-13 and Mut-7 showed a high surface hydrophobicity (78.9% and 83.5%) and medium autoaggregation ability (40.9% and 57.5%, respectively). The exposure of both isolates to the surface of the rat intestine increased the total number of lactic acid bacteria on the colon compartment, from 2.9 log CFU/cm to 4.4 log CFU/cm in Dad-13 treatment and to 3.86 log CFU/cm in Mut-7 treatment. The results indicate the ability of two to attach to the surface of the rat intestine. The number of indigenous in the colon also decreased when the compartment was exposed to Dad-13 and Mut-7, from 2.9 log CFU/cm to 1 log CFU/cm. Genomic analysis revealed that both strains have genes related to adhesion properties that could play an important role in increasing the adherence of probiotics to the intestinal mucosa such as gene encoding fibronectin-binding protein, chaperonin heat shock protein 33 (Hsp33) and genes related to the capsule and cell wall biosynthesis. Based on these findings, we believe that Dad-13 and Mut-7 have adhesion properties to the intestinal mucosa in the rat intestine model system. The present research will be essential to elucidate the molecular mechanism associated with adhesion in our two probiotic strains.
黏附能力被认为是益生菌菌株的选择标准之一。本研究的目的是确定两种候选益生菌Dad-13和Mut-7的黏附特性。评估内容包括使用微生物对碳氢化合物的黏附（MATH）法测定细胞表面疏水性、自聚集性，以及Dad-13和Mut-7对斯普拉格-道利大鼠肠黏膜的黏附，随后对这两种菌株进行基因组分析。Dad-13和Mut-7表现出较高的表面疏水性（分别为78.9%和83.5%）和中等的自聚集能力（分别为40.9%和57.5%）。将这两种分离株暴露于大鼠肠道表面后，结肠区的乳酸菌总数增加，在Dad-13处理组中从2.9 log CFU/cm增加到4.4 log CFU/cm，在Mut-7处理组中增加到3.86 log CFU/cm。结果表明这两种菌株能够附着于大鼠肠道表面。当结肠区暴露于Dad-13和Mut-7时，结肠中本土菌的数量也减少了，从2.9 log CFU/cm降至1 log CFU/cm。基因组分析表明，这两种菌株都有与黏附特性相关的基因，这些基因可能在增加益生菌对肠黏膜的黏附中发挥重要作用，例如编码纤连蛋白结合蛋白、伴侣热休克蛋白33（Hsp33）的基因以及与荚膜和细胞壁生物合成相关的基因。基于这些发现，我们认为Dad-13和Mut-7在大鼠肠道模型系统中对肠黏膜具有黏附特性。本研究对于阐明我们这两种益生菌菌株黏附相关的分子机制至关重要。
