Department of Medical Laboratory Science, College of Medicine and Health Sciences, Wachemo University, Hossana P.O. Box 667, Ethiopia.
Aklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa P.O. Box 1176, Ethiopia.
Int J Mol Sci. 2024 Sep 24;25(19):10251. doi: 10.3390/ijms251910251.
The diarrheagenic (DEC) is the major cause of diarrheal diseases in Africa, including Ethiopia. However, the genetic diversity of pathotypes found in Ethiopia has not been studied well. This study aimed to characterize potential DEC belonging to enteropathogenic (EPEC), Shiga toxin-producing (STEC), enteroaggregative (EAEC), enterotoxigenic (ETEC), and enteroinvasive (EIEC) pathotypes from stool specimens of patients attending primary healthcare units ( = 260) in Addis Ababa and Hossana using whole-genome sequencing. Real-time PCR assays were used to identify DEC isolates belonging to EPEC, STEC, EAEC, ETEC, and EIEC pathotypes, which were then subjected to whole-genome sequencing on the Illumina platform. Twenty-four whole-genome nucleotide sequences of DEC strains with good enough quality were analyzed for virulence-associated genes (VAGs), antibiotic resistance genes (ARGs), phylogenetic groups, serogroups, and sequence types. The majority (62.5%) of DEC isolates belonged to the phylogenetic group B1. The identified DEC isolates belonged to 21 different serogroups and 17 different sequence types. All tested DEC isolates carried multiple VAGs and ARGs. The findings highlight the high diversity in the population structure of the studied DEC isolates, which is important for designing targeted interventions to reduce the diarrheal burden in Ethiopia.
产毒性（DEC）是非洲（包括埃塞俄比亚）腹泻病的主要病因。然而，在埃塞俄比亚发现的各种血清型的遗传多样性尚未得到很好的研究。本研究旨在通过全基因组测序，从亚的斯亚贝巴和霍萨纳的初级保健单位就诊的患者的粪便标本中，对属于肠致病性大肠杆菌（EPEC）、志贺毒素产生大肠杆菌（STEC）、肠聚集性大肠杆菌（EAEC）、肠毒性大肠杆菌（ETEC）和肠侵袭性大肠杆菌（EIEC）血清型的潜在 DEC 进行特征描述（n = 260）。实时 PCR 检测用于鉴定属于 EPEC、STEC、EAEC、ETEC 和 EIEC 血清型的 DEC 分离株，然后在 Illumina 平台上对其进行全基因组测序。对 24 株 DEC 菌株的高质量全基因组核苷酸序列进行了分析，以研究与毒力相关的基因（VAGs）、抗生素耐药基因（ARGs）、进化群、血清群和序列类型。大多数（62.5%）DEC 分离株属于进化群 B1。鉴定出的 DEC 分离株属于 21 个不同的血清群和 17 个不同的序列类型。所有测试的 DEC 分离株都携带多种 VAGs 和 ARGs。这些发现强调了研究中 DEC 分离株群体结构的高度多样性，这对于设计有针对性的干预措施以减少埃塞俄比亚的腹泻负担非常重要。
