Prakash N, Ragavan B
Department of Biochemistry, PSG College of Arts and Science, Coimbatore-14, Tamilnadu, India.
Anc Sci Life. 2009 Apr;28(4):16-20.
In the present study various extracts of Cyperus esculentus L. such as acetone, 50% ethanol, chloroform and petroleum ether were evaluated for their antibacterial activity against several human pathogens such as Escherichia coli, Staphylococcus aureus, Salmonella sp, Klebsiella pneumoniae, Proteus vulgaris, Pseudomonas aeruginosa and Citrobacter freundii, by using disc diffusion method. The activity of the samples were compared with that of standard antibiotics. The qualitative phytochemical analysis was also carried out with all the extracts of the sample. Of all the extracts 50% ethanol and acetone extract were found to be rich in phytochemicals such as alkaloids, flavonoids, phenols, tannins, steroids, terpenoids and glycosides. Acetone extract showed highest inhibitory activity against S.aureus, ILpneumoniae and P.vulgaris. 50% ethanolic extract showed maximum activity against E.coli, S.aureus and Salmonella sp. Chloroform extract maximally inhibited the growth of S.aureus whereas, petroleum ether extract showed positive results against Salmonella sp respectively. All extracts were sensitive to C. freundii.
在本研究中,采用纸片扩散法评估了香附子的各种提取物,如丙酮提取物、50%乙醇提取物、氯仿提取物和石油醚提取物,对几种人类病原体的抗菌活性,这些病原体包括大肠杆菌、金黄色葡萄球菌、沙门氏菌属、肺炎克雷伯菌、普通变形杆菌、铜绿假单胞菌和弗氏柠檬酸杆菌。将样品的活性与标准抗生素的活性进行了比较。还对样品的所有提取物进行了定性植物化学分析。在所有提取物中,发现50%乙醇提取物和丙酮提取物富含生物碱、黄酮类化合物、酚类、单宁、甾体、萜类化合物和糖苷等植物化学物质。丙酮提取物对金黄色葡萄球菌、肺炎克雷伯菌和普通变形杆菌显示出最高的抑制活性。50%乙醇提取物对大肠杆菌、金黄色葡萄球菌和沙门氏菌属显示出最大活性。氯仿提取物对金黄色葡萄球菌的生长具有最大抑制作用,而石油醚提取物分别对沙门氏菌属显示出阳性结果。所有提取物对弗氏柠檬酸杆菌均敏感。