Kumar Raju Senthil, Moorthy Kannaiyan, Vinodhini Raja, Punitha Thambidurai
Department of Pharmaceutical Chemistry, Swamy Vivekanandha College of Pharmacy, Elayampalayam, Tiruchengode, Namakkal - 637 205, Tamil Nadu, India.
Afr J Tradit Complement Altern Med. 2013 Apr 12;10(3):518-25. eCollection 2013.
An in vitro antimicrobial activity and phytochemical analysis of various extracts of Indigofera trita L. viz. petroleum ether, chloroform, acetone, ethanol and aqueous extracts were carried out. A total of 21 microorganisms (19 bacteria and 2 fungal strains) were used for antimicrobial activity by disc diffusion method and a standard procedure was used to identify the phytochemical constituents. Petroleum ether extract showed moderate inhibitory activity against Staphylococcus aureus (14.40 mm), S. epidermidis (14.20 mm), Salmonella paratyphi A (12.80 mm), Streptococcus mutans (12.20 mm), Escherichia coli, Proteus vulgaris, S. typhi and Burkholderia cepacia (12.00 mm). The chloroform extract also showed antimicrobial activity against S. epidermidis (14.20 mm), S. typhimurium (12.60 mm), S. paratyphi A, S. brunei and Yersinia enterocolitica (12.00 mm). The acetone extract of I. trita showed considerable inhibitory activity against S. epidermidis (18.20 mm), S. typhimurium (14.60 mm), S. infantis (13.80 mm), S. aureus (13.40 mm), Y. enterocolitica (13.00 mm) and Enterobacter aerogenes (12.00 mm) were documented. Ethanol extract showed significant antimicrobial activity against S. epidermidis (18.60 mm), S. paratyphi A (14.60 mm), Y. enterocolitica (13.40 mm), S. typhi (12.40 mm), S. aureus, E. aerogenes, S. typhimurium and S. infantis (12.00 mm). Aqueous extract of I. trita considerably inhibited S. epidermidis (13.80 mm), S. paratyphi A and Y. enterocolitica (12.20 mm), E. aerogenes and Haemophilus parahaemolyticus (12.00 mm). All the five extracts showed a minimal antifungal activity when compared to antibacterial activity. The result revealed that the antimicrobial properties of I. trita might be associated with the presence of phenolic compounds, flavonoids, tannins, glycosides, saponins, phytosterols and alkaloids.
对木蓝（Indigofera trita L.）的各种提取物，即石油醚提取物、氯仿提取物、丙酮提取物、乙醇提取物和水提取物进行了体外抗菌活性和植物化学分析。采用纸片扩散法对总共21种微生物（19种细菌菌株和2种真菌菌株）进行抗菌活性测试，并使用标准程序鉴定植物化学成分。石油醚提取物对金黄色葡萄球菌（抑菌圈直径14.40毫米）、表皮葡萄球菌（14.20毫米）、副伤寒沙门氏菌A（12.80毫米）、变形链球菌（12.20毫米）、大肠杆菌、普通变形杆菌、伤寒沙门氏菌和洋葱伯克霍尔德菌（12.00毫米）表现出中等抑制活性。氯仿提取物对表皮葡萄球菌（14.20毫米）、鼠伤寒沙门氏菌（12.60毫米）、副伤寒沙门氏菌A、布鲁氏链球菌和小肠结肠炎耶尔森菌（12.00毫米）也表现出抗菌活性。木蓝的丙酮提取物对表皮葡萄球菌（18.20毫米）、鼠伤寒沙门氏菌（14.60毫米）、婴儿链球菌（13.80毫米）、金黄色葡萄球菌（13.40毫米）、小肠结肠炎耶尔森菌（13.00毫米）和气单胞菌（12.00毫米）表现出显著抑制活性。乙醇提取物对表皮葡萄球菌（18.60毫米）、副伤寒沙门氏菌A（14.60毫米）、小肠结肠炎耶尔森菌（13.40毫米）、伤寒沙门氏菌（12.40毫米）、金黄色葡萄球菌、气单胞菌、鼠伤寒沙门氏菌和婴儿链球菌（12.00毫米）表现出显著抗菌活性。木蓝的水提取物对表皮葡萄球菌（13.80毫米）、副伤寒沙门氏菌A和小肠结肠炎耶尔森菌（12.20毫米）和气单胞菌及副溶血性嗜血杆菌（12.00毫米）有显著抑制作用。与抗菌活性相比，所有这五种提取物均表现出最低限度的抗真菌活性。结果表明，木蓝的抗菌特性可能与酚类化合物、黄酮类化合物、单宁、糖苷、皂苷、植物甾醇和生物碱的存在有关。
