Centre of Biotechnology and Microbiology, University of Peshawar, Peshawar 25000, Pakistan.
J Tradit Chin Med. 2019 Dec;39(6):764-771.
To aim at preliminary phytochemical screening of Daphne mucronata and evaluate its antioxidant and antibacterial activities.
Preliminary phytochemical screening was conducted for the crude extracts using standard methods. Antioxidant properties of crude methanolic extracts, n-hexane, chloroform, ethyl acetate and aqueous fractions of leaves, roots and bark were evaluated following standard procedures. The antibacterial activities were checked against Acinetobacter baumannii (A. baumannii), Escherichia coli (E. coli), Pseudomonas aeruginosa (P. aeruginosa), Staphylococcus aureus (S. aureus), Morganella morganii (M. morganii) and vancomycin resistant S. aureus (VRSA).
Carbohydrates, saponins, steroids, phenols/ tannins, flavonoids and glycosides were present in different parts of Daphne mucronata. The extracts showed good antioxidant activity with EC50 values of 157.82-361.61 μg/mL. Methanolic extracts of roots showed good activity against A. baumannii (86.95%), E. coli (85.18%) and S. aureus (84.61%). Methanolic extracts of bark were active against A. bumanni (65.21%), M. morganii (65.21%) and E. coli (62.96%). Methanolic extracts of leaves showed good activity against A. bumanni (78.26%), E. coli (77.78%), P. aeruginosa (74.07%), S. aureus (73.07%), M. morganii (69.56%), VRSA (68%) and Proteus vulgaris (60%). The n-hexane fraction of roots was effective against A. bumanni (78.26%). Chlorofom fraction of roots showed moderate activity against A. bumanni (60.86%) and S. aureus (61.53%). Ethyl acetate fraction of roots showed moderate activity against A. bumanii (69.56%), E. coli (62.96%) and S. aureus (69.23%).
This study illustrates that Daphne mucronata possesses good antioxidant and antibacterial properties. The plant could be further exploited as potential natural antioxidant and as a new source of antimicrobials for treatment of various infections.
旨在对瑞香科荛花进行初步的植物化学筛选，并评价其抗氧化和抗菌活性。
采用标准方法对粗提取物进行初步的植物化学筛选。采用标准程序评价叶、根和树皮的粗甲醇提取物、正己烷、氯仿、乙酸乙酯和水提 fractions 的抗氧化性能。根据对鲍曼不动杆菌（A.baumannii）、大肠杆菌（E.coli）、铜绿假单胞菌（P.aeruginosa）、金黄色葡萄球菌（S.aureus）、摩氏摩根菌（M.morganii）和耐万古霉素金黄色葡萄球菌（VRSA）的抑制作用来检测抗菌活性。
碳水化合物、皂苷、类固醇、酚类/鞣质、类黄酮和糖苷存在于荛花的不同部位。提取物具有良好的抗氧化活性，EC50 值为 157.82-361.61μg/mL。根的甲醇提取物对 A.baumannii（86.95%）、E.coli（85.18%）和 S.aureus（84.61%）表现出良好的活性。树皮的甲醇提取物对 A.baumannii（65.21%）、M.morganii（65.21%）和 E.coli（62.96%）有活性。叶的甲醇提取物对 A.baumannii（78.26%）、E.coli（77.78%）、P.aeruginosa（74.07%）、S.aureus（73.07%）、M.morganii（69.56%）、VRSA（68%）和普通变形杆菌（60%）表现出良好的活性。根的正己烷部分对 A.baumannii（78.26%）有效。根的氯仿部分对 A.baumannii（60.86%）和 S.aureus（61.53%）表现出中等活性。根的乙酸乙酯部分对 A.baumannii（69.56%）、E.coli（62.96%）和 S.aureus（69.23%）表现出中等活性。
本研究表明荛花具有良好的抗氧化和抗菌性能。该植物可以进一步开发为潜在的天然抗氧化剂和治疗各种感染的新的抗菌药物来源。
