Nakamura K, Sato T, Kakizaki G
Department of Pathology, Sendai Shakaihoken Hospital, Japan.
Tohoku J Exp Med. 1990 Aug;161(4):283-301. doi: 10.1620/tjem.161.283.
The findings of electron microscopic observation of the intercellular junctions of pancreatic exocrine cells from male Wistar rats that had been given ethanol ad libitum in place of water are described. In control animals, numerous tight junctions having a distinct pentalamellar structure were observed in the well developed interdigitations of the baso-lateral domain of both the ductal and centroacinar cells. This type of junction differed structurally as well as topographically from the occluding junctions situated in the apical part of the lateral domain and exhibited a tri-lamellar rather than a penta-lamellar structure. Further, this type of junction was not observed in the interdigitations between the acinar and centroacinar cells or in those between acinar cells. These specialized membrane structures became hypertrophic after 1 and 2 months of ethanol ingestion. That is, the interdigitated cell processes became elongated but the distribution density of the tight junctions was similar to that in control animals, so that the total number of tight junctions increased. After 3 months of ethanol ingestion, the interdigitated cell processes atrophied, the intercellular spaces widened, and the number of tight junctions markedly decreased. Thereafter, these changes were observed continuously in ethanol-treated animals. However, the occluding and adhering junctions were not markedly affected by ethanol intake.
本文描述了对以乙醇随意替代水喂养的雄性Wistar大鼠胰腺外分泌细胞细胞间连接进行电子显微镜观察的结果。在对照动物中，在导管细胞和腺泡中心细胞基底外侧区域发育良好的指状交叉中观察到许多具有明显五片层结构的紧密连接。这种连接在结构和拓扑上与位于外侧区域顶端部分的封闭连接不同，呈现出三层而不是五层结构。此外，在腺泡细胞与腺泡中心细胞之间的指状交叉或腺泡细胞之间的指状交叉中未观察到这种连接。在摄入乙醇1个月和2个月后，这些特殊的膜结构变得肥大。也就是说，相互交错的细胞突起变长，但紧密连接的分布密度与对照动物相似，因此紧密连接的总数增加。摄入乙醇3个月后，相互交错的细胞突起萎缩，细胞间隙增宽，紧密连接的数量明显减少。此后，在乙醇处理的动物中持续观察到这些变化。然而，封闭连接和黏着连接并未受到乙醇摄入的明显影响。
