ADAS UK, School of Veterinary Medicine and Science, The University of Nottingham, Sutton Bonington, Leicestershire, UK.
Prion. 2012 Jul 1;6(3):302-8. doi: 10.4161/pri.20025.
Ovine scrapie and cervid chronic wasting disease can be transmitted in the absence of animal-to-animal contact, and environmental reservoirs of infectivity have been implicated in their spread and persistence. Investigating environmental factors that influence the interaction of disease-associated PrP with soils is imperative to understanding what is likely to be the complex role of soil in disease transmission. Here, we describe the effects of soil temperature on the binding/desorption and persistence of both ovine scrapie- and bovine BSE-PrP (TSE) . Binding of PrP (TSE) to a sandy loam soil at temperatures of 4°C, 8-12°C and 25-30°C demonstrated that an increase in temperature resulted in (1) a decrease in the amount of PrP (TSE) recovered after 24 h of interaction with soil, (2) an increase in the amount of N-terminal cleavage of the prion protein over 11 d and (3) a decrease in the persistence of PrP (TSE) on soil over an 18 mo period.
绵羊痒病和鹿慢性消耗病可在没有动物与动物接触的情况下传播，而且环境传染性储库与它们的传播和持续存在有关。研究影响疾病相关朊病毒与土壤相互作用的环境因素，对于理解土壤在疾病传播中可能具有的复杂作用至关重要。在这里，我们描述了土壤温度对绵羊痒病和牛朊病毒病-PrP（朊病毒病）结合/解吸和持久性的影响。在 4°C、8-12°C 和 25-30°C 的沙壤土温度下，PrP（朊病毒病）的结合表明，温度升高会导致：（1）与土壤相互作用 24 小时后回收的 PrP（朊病毒病）量减少；（2）在 11 天内朊病毒蛋白 N 端切割量增加；（3）在 18 个月的时间里，土壤中朊病毒病-PrP 的持久性降低。
