AGH University of Science and Technology, Faculty of Physics and Applied Computer Science, al. A. Mickiewicza 30, 30-059 Krakow, Poland.
Neurochem Int. 2012 Jul;61(2):156-65. doi: 10.1016/j.neuint.2012.04.018. Epub 2012 Apr 25.
Recent studies of Parkinson's disease indicate that dorsal motor nucleus of nerve vagus is one of the earliest brain areas affected by alpha-synuclein and Lewy bodies pathology. The influence of electrical stimulation of vagus nerve on elemental composition of dopamine related brain structures in rats is investigated. Synchrotron radiation based X-ray fluorescence was applied to the elemental micro-imaging and quantification in thin tissue sections. It was found that elements such as P, S, Cl, K, Ca, Fe, Cu, Zn, Se, Br and Rb are present in motor cortex, corpus striatum, nucleus accumbens, substantia nigra, ventral tectal area, and dorsal motor nucleus of vagus. The topographic analysis shows that macro-elements like P, S, Cl and K are highly concentrated within the fiber bundles of corpus striatum. In contrast the levels of trace elements like Fe and Zn are the lowest in these structures. It was found that statistically significant differences between the animals with electrical stimulation of vagus nerve and the control are observed in the left side of corpus striatum for P (p = 0.04), S (p = 0.02), Cl (p = 0.05), K (p = 0.02), Fe (p = 0.04) and Zn (p = 0.02). The mass fractions of these elements are increased in the group for which the electrical stimulation of vagus nerve was performed. Moreover, the contents of Ca (p = 0.02), Zn (p = 0.07) and Rb (p = 0.04) in substantia nigra of right hemisphere are found to be significantly lower in the group with stimulation of vagus nerve than in the control rats.
最近对帕金森病的研究表明，迷走神经背核是最早受α-突触核蛋白和路易体病理学影响的大脑区域之一。研究了迷走神经电刺激对大鼠与多巴胺相关的脑结构元素组成的影响。应用同步辐射 X 射线荧光对薄组织切片中的元素微成像和定量进行了研究。结果发现，P、S、Cl、K、Ca、Fe、Cu、Zn、Se、Br 和 Rb 等元素存在于运动皮层、纹状体、伏隔核、黑质、腹侧脑桥区和迷走神经背核。拓扑分析表明，P、S、Cl 和 K 等宏量元素在纹状体的纤维束中高度集中。相比之下，Fe 和 Zn 等微量元素在这些结构中的含量最低。研究发现，在电刺激迷走神经的动物和对照组之间，在左侧纹状体中观察到 P（p = 0.04）、S（p = 0.02）、Cl（p = 0.05）、K（p = 0.02）、Fe（p = 0.04）和 Zn（p = 0.02）的统计学显著差异。在进行迷走神经电刺激的组中，这些元素的质量分数增加。此外，右半球黑质中 Ca（p = 0.02）、Zn（p = 0.07）和 Rb（p = 0.04）的含量在刺激迷走神经的组中明显低于对照组大鼠。
