Department of Agronomy and Agricultural Extension, Faculty of Agriculture, University of Rajshahi, Rajshahi-6205, Bangladesh.
J Microbiol Biotechnol. 2012 May;22(5):585-91. doi: 10.4014/jmb.1107.07063.
The antagonistic effect of Trichoderma harzianum on a range of seed-borne fungal pathogens of wheat (viz. Fusarium graminearum, Bipolaris sorokiniana, Aspergillus spp., and Penicillium spp.) was assessed. The potential of T. harzianum as a biocontrol agent was tested in vitro and under field conditions. Coculture of the pathogens and Trichoderma under laboratory conditions clearly showed dominance of T. harzianum. Under natural conditions, biocontrol effects were also obtained against the test fungi. One month after sowing, field emergence (plant stand) was increased by 15.93% over that obtained with the control treatment, and seedling infection was reduced significantly. Leaf blight severity was decreased from 22 to 11 at the heading stage, 35 to 31 at the flowering stage, and 86 to 74 at the grain filling stage. At harvest, the number of tillers per plant was increased by 50%, the yield was increased by 31.58%, and the 1,000-seed weight was increased by 21%.
木霉属真菌（Trichoderma harzianum）对小麦种子带菌真菌病原体（禾谷镰刀菌（Fusarium graminearum）、旋孢腔菌（Bipolaris sorokiniana）、曲霉菌属（Aspergillus spp.）和青霉菌属（Penicillium spp.））的拮抗作用进行了评估。木霉属真菌作为生物防治剂的潜力在体外和田间条件下进行了测试。在实验室条件下，病原体和木霉属真菌的共培养清楚地表明木霉属真菌的优势地位。在自然条件下，对测试真菌也获得了生物防治效果。播种后一个月，与对照处理相比，田间出苗率（植物种群）增加了 15.93%，幼苗感染显著减少。在抽穗期，叶片枯萎严重程度从 22 降低到 11，在开花期从 35 降低到 31，在灌浆期从 86 降低到 74。收获时，每株分蘖数增加 50%，产量增加 31.58%，千粒重增加 21%。
