Department of Hepato-Biliary-Splenic Surgery, General Surgery, Shengjing Hospital, China Medical University, Shenyang 110004, China.
Dig Dis Sci. 2012 Sep;57(9):2340-6. doi: 10.1007/s10620-012-2182-8. Epub 2012 May 6.
At present, the relationship between Nanog expression and the biological behavior and prognosis of colorectal cancer is still unclear.
The purpose of this study was to evaluate the expression and regulatory effects of Nanog in colorectal cancer and the correlation between Nanog protein expression and the prognosis of patients with colorectal cancer.
The differential expression of genes between CD133+ tumor cells and CD133- tumor cells were detected using RT(2) Profiler™ PCR Array. The Nanog mRNA expression level was detected by RT-PCR and the protein level was detected using immunohistochemistry staining. The relationship between Nanog expression and clinicopathological parameters of colorectal cancer was determined.
Nanog were expressed significantly higher in CD133+ tumor cells compared to CD133- tumor cells. It was observed that 72 (20.00 %) of the 360 cases positively expressed Nanog. Univariate analyses indicated that Nanog expression was related to histological grade, lymph node metastasis, TNM stage, and liver metastasis (P = 0.005, 0.001, 0.001 and 0.012, respectively). Spearman correlation analysis showed that Nanog expression has a linear correlation to liver metastasis (P = 0.001). After conducting multivariate analysis, histological grade, TNM stage, and Nanog were found to be related to liver metastasis (P = 0.020, 0.01 and 0.001, respectively). In the Cox regression test, the histological grade, Lymph node metastasis, TNM stage, liver metastasis, and Nanog were detected as the independent prognostic factors (P = 0.02, 0.045, 0.01, 0.001 and 0.001, respectively).
Nanog protein may be a potential biomarker for postoperative liver metastasis of colorectal cancer.
目前，Nanog 表达与结直肠癌的生物学行为和预后的关系尚不清楚。
本研究旨在评估 Nanog 在结直肠癌中的表达及其调控作用，以及 Nanog 蛋白表达与结直肠癌患者预后的相关性。
采用 RT(2) Profiler™ PCR 阵列检测 CD133+肿瘤细胞与 CD133-肿瘤细胞之间基因的差异表达。采用 RT-PCR 检测 Nanog mRNA 表达水平，采用免疫组织化学染色检测 Nanog 蛋白表达水平。分析 Nanog 表达与结直肠癌临床病理参数的关系。
CD133+肿瘤细胞中 Nanog 的表达明显高于 CD133-肿瘤细胞。结果发现，360 例中有 72 例（20.00%）表达 Nanog 阳性。单因素分析表明，Nanog 表达与组织学分级、淋巴结转移、TNM 分期和肝转移有关（P=0.005、0.001、0.001 和 0.012）。Spearman 相关分析表明，Nanog 表达与肝转移呈线性相关（P=0.001）。多因素分析后发现，组织学分级、TNM 分期和 Nanog 与肝转移有关（P=0.020、0.01 和 0.001）。在 Cox 回归检验中，组织学分级、淋巴结转移、TNM 分期、肝转移和 Nanog 被检测为独立的预后因素（P=0.02、0.045、0.01、0.001 和 0.001）。
Nanog 蛋白可能是结直肠癌术后肝转移的潜在标志物。
