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Rapid and efficient technique for the production of condensed DNA and RNA nanoparticles using thermal cycling.

作者信息

Danilevich V N

机构信息

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997 Russia.

出版信息

Dokl Biochem Biophys. 2012 Mar-Apr;443:71-5. doi: 10.1134/S1607672912020044. Epub 2012 May 5.

DOI:10.1134/S1607672912020044
PMID:22562629
Abstract
摘要

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[Condensed DNA particles formed in PCR with plasmid DNA: electron microscopy study].[聚合酶链反应中与质粒DNA形成的浓缩DNA颗粒：电子显微镜研究]
Bioorg Khim. 2010 Jul-Aug;36(4):535-46. doi: 10.1134/s1068162010040084.
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[Micro- and nanoparticles of condensed DNA formed in a PCR with yeast genomic DNA as a template. Electron microscopy data].[以酵母基因组DNA为模板通过聚合酶链反应形成的浓缩DNA的微米和纳米颗粒。电子显微镜数据]
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[Characteristics of microspheres formed with bacterial genomic DNA and plasmid templates in the process of polymerase chain reaction].
[聚合酶链反应过程中细菌基因组DNA和质粒模板形成的微球的特征]
Mikrobiologiia. 2009 May-Jun;78(3):369-80.
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[Microparticles from coupled DNA formed in the process of polymerase chain reaction].[聚合酶链反应过程中形成的偶联DNA微粒]
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mRNA-based gene transfer as a tool for gene and cell therapy.基于信使核糖核酸的基因转移作为基因和细胞治疗的一种工具。
Curr Opin Mol Ther. 2007 Oct;9(5):423-31.
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Delivery strategies for siRNA-mediated gene silencing.用于小干扰RNA介导的基因沉默的递送策略。
Curr Drug Deliv. 2006 Apr;3(2):147-5. doi: 10.2174/156720106776359159.
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DNA condensation by multivalent cations.多价阳离子介导的DNA凝聚
Biopolymers. 1997;44(3):269-82. doi: 10.1002/(SICI)1097-0282(1997)44:3<269::AID-BIP6>3.0.CO;2-T.
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DNA condensation.DNA凝聚
Curr Opin Struct Biol. 1996 Jun;6(3):334-41. doi: 10.1016/s0959-440x(96)80052-2.
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Aggregation of melted DNA by divalent metal ion-mediated cross-linking.通过二价金属离子介导的交联使熔化的DNA聚集。
Biophys J. 1995 Dec;69(6):2642-8. doi: 10.1016/S0006-3495(95)80134-7.
10
Condensation of supercoiled DNA induced by MnCl2.氯化锰诱导的超螺旋DNA凝聚
Biophys J. 1994 Oct;67(4):1678-81. doi: 10.1016/S0006-3495(94)80641-1.