Pancreatobiliary Cancer Clinic, Department of Surgery, Gangnam Severance Hospital, Yonsei University Health System, Seoul, Korea.
J Korean Med Sci. 2012 May;27(5):495-9. doi: 10.3346/jkms.2012.27.5.495. Epub 2012 Apr 25.
Angiogenesis is essential for tumor growth and metastasis. Currently, the Chalkley assay with CD34 immunostaining is the proposed standard method for angiogenesis quantification in solid tumor sections. The purpose of this study was to evaluate the expression of CD34 and its prognostic significance using the Chalkley method in node negative carcinoma of the ampulla of Vater. Between January 1997 and December 2006, 56 node negative patients who had curative resection for carcinoma of the ampulla of Vater were retrospectively reviewed. The Chalkley count was expressed as the mean value of the three counts for each tumor and further divided into two groups according to the mean value of the Chalkley count: low < 4 or high ≥ 4. The mean Chalkley count value was 4.0 (± 3.1). In the low Chalkley group, the 1- and 3-yr recurrence rates were 18.3%, 47.6% respectively; in the high Chalkley group, the 1- and 3-yr recurrence rates were 26.5% and 60.6% respectively. Only high Chalkley count had statistical significance as a factor in recurrence of node negative ampulla of Vater carcinoma. Assessment of angiogenesis may have an important role in the prognostic evaluation of node negative cancer of the ampulla of Vater.
血管生成对于肿瘤的生长和转移至关重要。目前，CD34 免疫染色的 Chalkley 检测被认为是实体瘤切片中血管生成定量的标准方法。本研究旨在评估 CD34 的表达及其在壶腹癌中作为无淋巴结转移癌的预后意义。1997 年 1 月至 2006 年 12 月，回顾性分析了 56 例经根治性切除术治疗的无淋巴结转移壶腹癌患者。Chalkley 计数表示每个肿瘤的三个计数的平均值，并根据 Chalkley 计数的平均值进一步分为两组：低 < 4 或高≥ 4。Chalkley 计数的平均值为 4.0（±3.1）。在低 Chalkley 组中，1 年和 3 年的复发率分别为 18.3%和 47.6%；在高 Chalkley 组中，1 年和 3 年的复发率分别为 26.5%和 60.6%。只有高 Chalkley 计数是无淋巴结转移壶腹癌复发的一个有统计学意义的因素。血管生成的评估可能在无淋巴结转移壶腹癌的预后评估中具有重要作用。
