van Diest P J, Zevering J P, Zevering L C, Baak J P
Department of Pathology, Free University Hospital, Amsterdam, The Netherlands.
Pathol Res Pract. 1995 Feb;191(1):25-30. doi: 10.1016/S0344-0338(11)80918-0.
Primary ovarian carcinomas of 49 patients have been used to study the relation between microvessel counts and prognosis. All tumours were of the common epithelial type, FIGO 3 or 4, were treated by debulking surgery and cisplatin based chemotherapy. Primary tumour tissue obtained at surgery was embedded in paraffin, cut at 4 microns and stained with Ulex to accentuate the microvessels. The number of microvessels per unit area was counted according to a standardized protocol at a magnification of x 250 in 4 fields, which showed high field (R = 0.98) and acceptable case reproducibility (R = 0.70). Microvessell counts did not show associations with other prognostic variables such as FIGO stage, bulky disease-or-not, differentiation grade, DNA ploidy, volume percentage epithelium, mitotic activity index and mean nuclear area. In survival analysis, a tendency for worse prognosis with higher microvessel counts was found, although statistical significance was not reached (p = 0.25). Correction for volume percentage epithelium did not improve this result. Multivariate survival analysis did not reveal additional prognostic value of microvessel counts to other prognostic factors such as FIGO stage, bulky disease-or-not, volume percentage epithelium, mitotic activity index and mean and SD of nuclear area. In conclusion, although a tendency for worse survival with higher microvessel counts has been found, vascularity does not seem to have a significant impact on survival of adequately debulked advanced ovarian cancer patients treated with cisplatin.
对49例原发性卵巢癌患者进行研究，以探讨微血管计数与预后之间的关系。所有肿瘤均为常见上皮类型，国际妇产科联盟（FIGO）分期为3或4期，均接受了肿瘤细胞减灭术和顺铂化疗。手术获取的原发性肿瘤组织用石蜡包埋，切成4微米厚的切片，并用荆豆凝集素染色以突出微血管。按照标准化方案，在放大250倍的情况下，在4个视野中计数每单位面积的微血管数量，视野显示出高相关性（R = 0.98）和可接受的病例重复性（R = 0.70）。微血管计数与其他预后变量如FIGO分期、有无大块肿瘤、分化程度、DNA倍体、上皮体积百分比、有丝分裂活性指数和平均核面积均无关联。在生存分析中，发现微血管计数越高，预后越差的趋势，尽管未达到统计学显著性（p = 0.25）。校正上皮体积百分比并未改善这一结果。多因素生存分析未显示微血管计数相对于其他预后因素（如FIGO分期、有无大块肿瘤、上皮体积百分比、有丝分裂活性指数以及核面积的均值和标准差）具有额外的预后价值。总之，尽管已发现微血管计数越高生存越差的趋势，但血管生成似乎对接受顺铂治疗且肿瘤细胞减灭充分的晚期卵巢癌患者的生存没有显著影响。
