Dandekar Thomas, Astrid Fieselmann, Jasmin Popp, Hensel Michael
Department of Bioinformatics, Biocenter, University of Würzburg, Würzburg, Germany.
Front Microbiol. 2012 May 3;3:164. doi: 10.3389/fmicb.2012.00164. eCollection 2012.
The infectious intracellular lifestyle of Salmonella enterica relies on the adaptation to nutritional conditions within the Salmonella-containing vacuole (SCV) in host cells. We summarize latest results on metabolic requirements for Salmonella during infection. This includes intracellular phenotypes of mutant strains based on metabolic modeling and experimental tests, isotopolog profiling using (13)C-compounds in intracellular Salmonella, and complementation of metabolic defects for attenuated mutant strains towards a comprehensive understanding of the metabolic requirements of the intracellular lifestyle of Salmonella. Helpful for this are also genomic comparisons. We outline further recent studies and which analyses of intracellular phenotypes and improved metabolic simulations were done and comment on technical required steps as well as progress involved in the iterative refinement of metabolic flux models, analyses of mutant phenotypes, and isotopolog analyses. Salmonella lifestyle is well-adapted to the SCV and its specific metabolic requirements. Salmonella metabolism adapts rapidly to SCV conditions, the metabolic generalist Salmonella is quite successful in host infection.
肠炎沙门氏菌的传染性细胞内生活方式依赖于对宿主细胞内含沙门氏菌液泡（SCV）中营养条件的适应。我们总结了感染期间沙门氏菌代谢需求的最新研究结果。这包括基于代谢建模和实验测试的突变菌株的细胞内表型、使用（13）C化合物对细胞内沙门氏菌进行的同位素异构体分析，以及针对减毒突变菌株代谢缺陷的互补作用，以全面了解沙门氏菌细胞内生活方式的代谢需求。基因组比较对此也有帮助。我们概述了近期的进一步研究，以及所进行的细胞内表型分析和改进的代谢模拟，并对代谢通量模型的迭代优化、突变体表型分析和同位素异构体分析所需的技术步骤以及所取得的进展进行了评论。沙门氏菌的生活方式很好地适应了SCV及其特定的代谢需求。沙门氏菌的代谢能迅速适应SCV条件，这种代谢多面手沙门氏菌在宿主感染中相当成功。
