College of Life Science, Sichuan Agricultural University, Xinkang Road 46, Ya'an, Sichuan 625014, China.
Department of Biological Science, College of Life Science, Sichuan Normal University, Chengdu, Sichuan 610101, China.
J Agric Food Chem. 2020 Nov 25;68(47):13685-13696. doi: 10.1021/acs.jafc.0c03037. Epub 2020 Nov 10.
Tartary buckwheat () is rich in flavonols, which are thought to be highly beneficial for human health. However, little is known about the regulatory mechanism of flavonol biosynthesis in Tartary buckwheat. In this study, we identified and characterized a novel SG7 R2R3-MYB transcription factor in Tartary buckwheat, FtMYB6. We showed that is located in the nucleus and acts as a transcriptional activator. The promoter showed strong spatiotemporal specificity and was induced by light. The expression of showed a significant correlation with rutin accumulation in the roots, stems, leaves, and flowers. Overexpression of in transgenic Tartary buckwheat hairy roots and tobacco () plants significantly increased the accumulation of flavonols. In transient luciferase (LUC) activity assay, FtMYB6 promoted the activity of and promoters and inhibited the activity of the promoter. Collectively, our results suggest that FtMYB6 promotes flavonol biosynthesis by activating and expression.
鞑靼荞麦富含类黄酮，被认为对人体健康非常有益。然而，关于鞑靼荞麦中类黄酮生物合成的调控机制知之甚少。在这项研究中，我们在鞑靼荞麦中鉴定和表征了一个新的 SG7 R2R3-MYB 转录因子 FtMYB6。我们表明， 位于细胞核内，作为转录激活因子发挥作用。 启动子表现出强烈的时空特异性，并受光诱导。 的表达与根、茎、叶和花中芦丁的积累呈显著相关性。在转基因鞑靼荞麦毛状根和烟草（）植株中过表达 ，显著增加了类黄酮的积累。在瞬时荧光素酶（LUC）活性测定中，FtMYB6 促进 、 和 启动子的活性，并抑制 启动子的活性。总之，我们的研究结果表明，FtMYB6 通过激活 、 和 的表达来促进类黄酮的生物合成。
