锰(II)五氮杂大环过氧化物歧化酶类似物对人精子线粒体功能和运动性有益吗？
Is manganese (II) pentaazamacrocyclic superoxide dismutase mimic beneficial for human sperm mitochondria function and motility?
机构信息
Department of Physiology, Institute for Biological Research Sinisa Stankovic, University of Belgrade, Belgrade, Serbia.
出版信息
Antioxid Redox Signal. 2013 Jan 10;18(2):170-8. doi: 10.1089/ars.2012.4684. Epub 2012 Jun 12.
Abstract
Mitochondria play an important role in sperm cell maturation and function. Here, we examined whether (and how) changes in sperm redox milieu affect the functional status of sperm mitochondria, that is, sperm functionality. Compared with the control, incubation in Tyrode's medium for 3 h, under noncapacitating conditions, decreased sperm motility, the amount of nitric oxide ((•)NO), the number of MitoTracker(®) Green FM (MT-G) positive mitochondria, and the expression of complexes I and IV of the mitochondrial respiratory chain. In turn, superoxide dismutase (SOD) mimic (M40403) treatment restored/increased these parameters, as well as the expression of endothelial nitric oxide synthase, manganese SOD, and catalase. These data lead to the hypothesis that M40403 improves mitochondrial functional state and motility of spermatozoa, as well as (•)NO might be involved in the observed effects of the mimic.
摘要
线粒体在精子细胞成熟和功能中发挥着重要作用。在这里，我们研究了精子氧化还原环境的变化是否（以及如何）影响精子线粒体的功能状态，即精子功能。与对照组相比，在非诱导条件下，Tyrode 培养基孵育 3 小时会降低精子的运动能力、一氧化氮（•）NO 的含量、MitoTracker（®）Green FM（MT-G）阳性线粒体的数量以及线粒体呼吸链复合物 I 和 IV 的表达。相反，超氧化物歧化酶（SOD）模拟物（M40403）处理恢复/增加了这些参数，以及内皮型一氧化氮合酶、锰 SOD 和过氧化氢酶的表达。这些数据表明，M40403 可改善精子线粒体的功能状态和运动能力，并且（•）NO 可能参与了模拟物观察到的效果。
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