Castleton Patience E, Deluao Joshua C, Sharkey David J, McPherson Nicole O
Freemasons Centre for Male Health and Wellbeing, The University of Adelaide, Adelaide 5005, Australia.
Robinson Research Institute, The University of Adelaide, Adelaide 5005, Australia.
Antioxidants (Basel). 2022 Jan 28;11(2):264. doi: 10.3390/antiox11020264.
Oxidative stress and elevated levels of seminal and sperm reactive oxygen species (ROS) may contribute to up to 80% of male infertility diagnosis, with sperm ROS concentrations at fertilization important in the development of a healthy fetus and child. The evaluation of ROS in semen seems promising as a potential diagnostic tool for male infertility and male preconception care with a number of clinically available tests on the market (MiOXSYS, luminol chemiluminescence and OxiSperm). While some of these tests show promise for clinical use, discrepancies in documented decision limits and lack of cohort studies/clinical trials assessing their benefits on fertilization rates, embryo development, pregnancy and live birth rates limit their current clinical utility. In this review, we provide an update on the current techniques used for analyzing semen ROS concentrations clinically, the potential to use of ROS research tools for improving clinical ROS detection in sperm and describe why we believe we are likely still a long way away before semen ROS concentrations might become a mainstream preconception diagnostic test in men.
氧化应激以及精液和精子中活性氧（ROS）水平升高可能导致高达80%的男性不育诊断病例，受精时精子ROS浓度对健康胎儿和儿童的发育至关重要。精液中ROS的评估作为男性不育和男性孕前保健的潜在诊断工具似乎很有前景，市场上有许多临床可用的检测方法（MiOXSYS、鲁米诺化学发光法和OxiSperm）。虽然其中一些检测方法显示出临床应用前景，但记录的决策限存在差异，且缺乏评估其对受精率、胚胎发育、妊娠和活产率影响的队列研究/临床试验，限制了它们目前的临床应用价值。在本综述中，我们提供了临床上用于分析精液ROS浓度的当前技术的最新情况，利用ROS研究工具改善精子临床ROS检测的潜力，并描述了为什么我们认为在精液ROS浓度可能成为男性孕前主流诊断测试之前，我们可能还有很长一段路要走。
