Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University School of Medicine, New Haven, CT, USA.
Am J Reprod Immunol. 2012 Aug;68(2):146-53. doi: 10.1111/j.1600-0897.2012.01148.x. Epub 2012 May 8.
Toll-like receptor-4 (TLR-4) protects against Gram-negative bacteria expressed lipopolysaccharide and 'danger signals' from injured or dying cells. Although decidual cells (DCs) and interstitial trophoblasts (ITs) are in close contact, TLR-4 has been studied extensively only in ITs.
Formalin-fixed, paraffin-embedded serial sections of endometrium in follicular and luteal phases and deciduas from first and second trimester elective terminations and third trimester normal deliveries were immunostained for TLR-4, trophoblast-specific cytokeratin, and DC-specific vimentin. HSCORE assessed TLR-4 immunostaining in DCs versus ITs.
TLR-4 HSCORES were significantly higher in: (i) first trimester DCs than luteal phase pre-decidual stromal cells; (ii) first and third versus second trimester DCs, but similar between third trimester deciduas parietalis and basalis; (iii) first versus second trimester ITs; (iv) DCs versus ITs across gestation.
Higher TLR-4 in DCs than ITs suggests DCs as primary targets for Gram-negative bacteria and/or inflammation-related danger signals.
Toll 样受体-4（TLR-4）可保护机体免受革兰氏阴性菌表达的脂多糖和受损或死亡细胞释放的“危险信号”的侵害。尽管蜕膜细胞（DCs）和间质滋养层细胞（ITs）紧密接触，但 TLR-4 仅在 ITs 中得到了广泛研究。
对卵泡期和黄体期子宫内膜以及第一和第二孕期选择性终止妊娠和第三孕期正常分娩的蜕膜进行福尔马林固定、石蜡包埋的连续切片，用 TLR-4、滋养层特异性细胞角蛋白和 DC 特异性波形蛋白进行免疫染色。HSCORE 评估了 DC 与 IT 中 TLR-4 的免疫染色。
TLR-4 HSCORE 在以下情况中显著更高：（i）早孕期 DC 高于黄体期蜕膜基质细胞；（ii）早孕期和第三孕期 DC 高于中孕期 DC，但第三孕期胎盘壁蜕膜和底蜕膜之间无差异；（iii）早孕期 DC 高于中孕期 IT；（iv）整个孕期 DC 高于 IT。
与 IT 相比，DC 中 TLR-4 水平更高，提示 DC 是革兰氏阴性菌和/或与炎症相关的危险信号的主要靶标。
