Chiva Andreea
Department of Clinical Chemistry, University Emergency Hospital, Bucharest, Romania.
J Med Life. 2011 Aug 15;4(3):228-33. Epub 2011 Aug 25.
Dry eye is the most prevalent condition seen by the ophthalmologist, in particular in elderly. The identification of new common risk factors (computer use and contact lens wear) extends the disease among the young people. The early diagnosis of dry eye is essential, but difficult, because the biochemical changes in tear film usually occur before any detectable signs. Due its advantages, electrophoresis of tear proteins could be an important tool for diagnosis of tear film impairment in high risk groups for dry eye.
The role of tear proteins electrophoresis in early diagnosis of dry eye related to computer use and contact lens wear, as well as the biochemical changes in these high risk groups are presented.
This review will summarize the actual data concerning the electrophoretic changes of tear proteins in computer users and contact lens wearers, two common high risk groups for dry eye.
Electrophoresis of tear proteins using automated system Hyrys-Hydrasys SEBIA France is an important tool for early diagnosis of tear film alterations and monitoring of therapy. The quantification of many proteins in a single analysis using a small quantity of unconcentrated reflex tears is the main advantage of this technique. Electrophoresis of tear proteins should became a prerequisite, in particular for computer users less than 3 h/day, as well as at prescribing contact lenses.
干眼症是眼科医生最常遇到的病症，在老年人中尤为常见。新的常见风险因素（使用电脑和佩戴隐形眼镜）的发现使得年轻人中患干眼症的人数增多。干眼症的早期诊断至关重要，但却很困难，因为泪膜的生化变化通常在任何可检测到的体征出现之前就已发生。由于其优势，泪液蛋白质电泳可能成为诊断干眼症高危人群泪膜损伤的重要工具。
介绍泪液蛋白质电泳在与使用电脑和佩戴隐形眼镜相关的干眼症早期诊断中的作用，以及这些高危人群中的生化变化。
本综述将总结有关电脑使用者和隐形眼镜佩戴者（干眼症的两个常见高危人群）泪液蛋白质电泳变化的实际数据。
使用法国SEBIA公司的Hyrys-Hydrasys自动化系统进行泪液蛋白质电泳是早期诊断泪膜改变和监测治疗的重要工具。该技术的主要优点是使用少量未浓缩的反射泪液在单次分析中对多种蛋白质进行定量。泪液蛋白质电泳应成为一项先决条件，特别是对于每天使用电脑少于3小时的人群以及在开具隐形眼镜处方时。
