Department of Environmental Health Sciences, Mario Negri Institute for Pharmacological Research, 20156 Milan, Italy.
J Anal Methods Chem. 2012;2012:719010. doi: 10.1155/2012/719010. Epub 2012 Mar 18.
Perfluorooctane sulfonate (PFOS) and perfluorooctanoic (PFOA) acid are persistent contaminants which can be found in environmental and biological samples. A new and fast analytical method is described here for the analysis of these compounds in the edible part of fish samples. The method uses a simple liquid extraction by sonication, followed by a direct determination using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The linearity of the instrumental response was good, with average regression coefficients of 0.9971 and 0.9979 for PFOS and PFOA, respectively, and the coefficients of variation (CV) of the method ranged from 8% to 20%. Limits of detection (LOD) were 0.04 ng/g for both the analytes and recoveries were 90% for PFOS and 76% for PFOA. The method was applied to samples of homogenized fillets of wild and farmed fish from the Mediterranean Sea. Most of the samples showed little or no contamination by perfluorooctane sulfonate and perfluorooctanoic acid, and the highest concentrations detected among the fish species analyzed were, respectively, 5.96 ng/g and 1.89 ng/g. The developed analytical methodology can be used as a tool to monitor and to assess human exposure to perfluorinated compounds through sea food consumption.
全氟辛烷磺酸 (PFOS) 和全氟辛酸 (PFOA) 是持久性污染物，可在环境和生物样本中发现。本文描述了一种新的快速分析方法，用于分析鱼类样本可食用部分中这些化合物的含量。该方法采用简单的超声液液萃取，然后使用液相色谱-串联质谱法 (LC-MS/MS) 直接测定。仪器响应的线性度良好，PFOS 和 PFOA 的平均回归系数分别为 0.9971 和 0.9979，方法的变异系数 (CV) 范围为 8%至 20%。两种分析物的检出限 (LOD) 均为 0.04 ng/g，回收率分别为 PFOS 的 90%和 PFOA 的 76%。该方法应用于来自地中海的野生和养殖鱼类鱼片的均质样本。大多数样本显示出很少或没有全氟辛烷磺酸和全氟辛酸污染，在所分析的鱼类物种中，检测到的最高浓度分别为 5.96 ng/g 和 1.89 ng/g。所开发的分析方法可作为一种工具，用于通过海产品消费监测和评估人类接触全氟化合物的情况。
