Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, Japan.
FEBS Lett. 2012 Apr 5;586(7):1055-61. doi: 10.1016/j.febslet.2012.03.003. Epub 2012 Mar 10.
Crocin is an apocarotenoid glycosyl ester accumulating in fruits of Gardenia jasminoides and used as a food coloring and nutraceutical. For the first time, the two glucosyltransferases UGT75L6 and UGT94E5 that sequentially mediate the final glucosylation steps in crocin biosynthesis in G. jasminoides have been identified and functionally characterized. UGT75L6 preferentially glucosylates the carboxyl group of crocetin yielding crocetin glucosyl esters, while UGT94E5 glucosylates the 6' hydroxyl group of the glucose moiety of crocetin glucosyl esters. The expression pattern of neither UGT75L6 nor UGT94E5 correlated with the pattern of crocin accumulation in G. jasminoides.
藏红花素是一种在栀子果实中积累的类胡萝卜素糖苷酯，可用作食品着色剂和营养保健品。首次鉴定并功能表征了栀子中催化藏红花素生物合成最后两步糖基化反应的两个葡萄糖基转移酶 UGT75L6 和 UGT94E5。UGT75L6 优先将 crocetin 的羧基糖基化生成 crocetin 葡萄糖基酯，而 UGT94E5 则将 crocetin 葡萄糖基酯中葡萄糖部分的 6' 羟基糖基化。UGT75L6 和 UGT94E5 的表达模式均与栀子中藏红花素的积累模式无关。
