Key Laboratory of Propagation and Cultivation on Medicinal Plants, College of Agriculture, Guizhou University, Guizhou 550025, China.
Institute of Medicinal Plant Development, Chinese Academy of Medical Science, Peking Union Medical College, Beijing 100193, China.
Biomed Res Int. 2020 Apr 6;2020:2903861. doi: 10.1155/2020/2903861. eCollection 2020.
Crocins, enriched in fruits, have a pharmacological activity against central nervous system diseases, cardiovascular diseases, and cancer cell growth. The biosynthesis of crocins has been widely explored, but its regulatory mechanism remains unknown. Here, the basic helix-loop-helix (bHLH) transcription factors related to crocin biosynthesis were systematically identified on the basis of the genome of . A total of 95 transcription factor genes were identified, and their phylogenetic analysis indicated that they could be classified into 23 subfamilies. The combination of gene-specific expression patterns, the coexpression analysis of biosynthesis genes, and the analysis of promoter sequences in crocin biosynthesis pathways suggested that nine in might negatively regulate crocin biosynthesis. This study laid a foundation for understanding the regulatory mechanism of crocin biosynthesis and the improvement and breeding of varieties.
藏红花中的藏红花苷具有防治中枢神经系统疾病、心血管疾病和癌细胞生长的药理活性。藏红花苷的生物合成已经得到了广泛的研究，但它的调控机制尚不清楚。本研究基于基因组信息，系统地鉴定了与藏红花苷生物合成相关的基本螺旋-环-螺旋（bHLH）转录因子。共鉴定到 95 个转录因子基因，其系统发育分析表明它们可以分为 23 个亚家族。藏红花苷生物合成途径中生物合成基因的共表达分析、基因特异性表达模式的组合以及启动子序列分析表明，9 个可能负调控藏红花苷生物合成。该研究为了解藏红花苷生物合成的调控机制以及提高和培育优良品种奠定了基础。
