Jiangxi Academy of Forestry, Nanchang 330032, China.
Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, 159 Longpan Road, Nanjing 210037, China.
Int J Mol Sci. 2022 Jun 5;23(11):6321. doi: 10.3390/ijms23116321.
Crocins are valuable bioactive components of gardenia fruit, and their biosynthesis and accumulation have attracted widespread interest. Studies have investigated the biosynthesis and accumulation of crocin based on Illumina sequencing, but there is a lack of reports based on full-length transcriptome sequencing. Utilising SMRT sequencing and high-performance liquid chromatography (HPLC), we explored crocin biosynthesis and accumulation in the fruit of . HPLC analysis showed that crocins specifically exist in fruit and that the content of crocins increases gradually during fruit development. SMRT sequencing generated 46,715 high-quality full-length isoforms, including 5230 novel isoforms that are not present in the genome. Furthermore, a total of 46 genes and 91 lncRNAs were involved in the biosynthesis and accumulation of crocin. The qRT-PCR indicated that genes involved in crocin biosynthesis reached a peak in the NOV stage. These findings contributed to our understanding of crocin biosynthesis and accumulation.
藏红花素是栀子果实中具有重要价值的生物活性成分，其生物合成和积累受到广泛关注。研究人员基于 Illumina 测序对藏红花素的生物合成和积累进行了研究，但缺乏基于全长转录组测序的相关报道。本研究利用 SMRT 测序和高效液相色谱（HPLC）技术，探索了栀子果实中藏红花素的生物合成和积累过程。HPLC 分析表明，藏红花素特异性存在于果实中，且其含量在果实发育过程中逐渐增加。SMRT 测序共获得 46715 条高质量全长异构体，其中包括 5230 条在 基因组中不存在的新异构体。此外，共有 46 个基因和 91 个 lncRNA 参与了藏红花素的生物合成和积累。qRT-PCR 分析表明，参与藏红花素生物合成的基因在 NOV 期达到峰值。这些发现有助于我们深入了解藏红花素的生物合成和积累机制。
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