School of Primary Health Care, Monash University, Melbourne, Vic., Australia.
Cells Tissues Organs. 2012;196(4):339-52. doi: 10.1159/000335475. Epub 2012 May 8.
Understanding adaption to load is essential for prevention and treatment of tendinopathy/tendinosis. Cytokine release in response to load is one mechanism involved in mechanotransduction. The cytokine tumor necrosis factor alpha (TNF-α) is implicated in tendinosis and can induce apoptotic effects via tumor necrosis factor receptor 1 (TNFR1). The complete absence of information concerning the TNF-α system in Achilles tendon is a limitation as mid-portion Achilles tendinosis is very frequent.
To examine expression patterns of TNF-α and its two receptors (TNFR1 and TNFR2) in human Achilles tendinosis and control tissue and to biochemically confirm the presence of TNF-α in tendinosis tissue.
TNF-α and TNFR1 mRNA were detected via in situ hybridization. TNF-α, TNFR1, and TNFR2 were demonstrated immunohistochemically. Apoptosis markers were utilized. ELISA was used to detect TNF-α.
TNF-α and TNFR1 mRNA was detected in tenocytes of both tendinosis and control tendons. Tenocytes from both groups displayed specific immunoreactions for TNF-α, TNFR1, and TNFR2. The widened/rounded tenocytes of tendinosis samples exhibited the most intense immunoreactions. Apoptosis was detected in only a subpopulation of the tenocytes in tendinosis tissue. TNF-α was measurable in tendinosis tissue. Inflammatory cells were not seen.
This is the first evidence of the existence of the TNF-α system in the human Achilles tendon. Findings are confirmed at mRNA and protein levels as well as biochemically. The TNF-α system was in principle confined to the tenocytes. The connection between tenocyte morphology and the expression pattern of TNF-α, TNFR1, and TNFR2 suggests that the TNF-α system may be involved in tenocyte activation in Achilles tendinosis.
检测肿瘤坏死因子-α（TNF-α）及其两种受体（TNFR1 和 TNFR2）在人类跟腱腱病和正常组织中的表达模式，并通过生物化学方法证实腱病组织中 TNF-α的存在。
通过原位杂交检测 TNF-α 和 TNFR1mRNA 的表达，免疫组织化学检测 TNF-α、TNFR1 和 TNFR2 的表达。利用凋亡标志物进行检测。ELISA 用于检测 TNF-α。
TNF-α 和 TNFR1mRNA 在腱病和正常肌腱的腱细胞中均有检测到。两组的腱细胞均显示出 TNF-α、TNFR1 和 TNFR2 的特异性免疫反应。腱病样本中变宽/变圆的腱细胞表现出最强的免疫反应。凋亡仅在腱病组织中的一部分腱细胞中检测到。TNF-α 在腱病组织中可测量。未观察到炎症细胞。
这是 TNF-α 系统在人类跟腱中存在的首个证据。研究结果在 mRNA 和蛋白质水平以及生物化学水平上得到了证实。TNF-α 系统原则上局限于腱细胞。腱细胞形态与 TNF-α、TNFR1 和 TNFR2 的表达模式之间的联系表明，TNF-α 系统可能参与跟腱腱病中腱细胞的激活。
