聚腺苷酸化酶基本 N 端的新相互作用，可调节酿酒酵母中的聚腺苷酸化添加。
Novel interactions at the essential N-terminus of poly(A) polymerase that could regulate poly(A) addition in Saccharomyces cerevisiae.
机构信息
Tufts School of Medicine and the Sackler Graduate School of Biomedical Sciences, Boston, MA 02111, USA.
出版信息
FEBS Lett. 2012 Apr 24;586(8):1173-8. doi: 10.1016/j.febslet.2012.03.036. Epub 2012 Mar 24.
Abstract
Addition of poly(A) to the 3' ends of cleaved pre-mRNA is essential for mRNA maturation and is catalyzed by Pap1 in yeast. We have previously shown that a non-viable Pap1 mutant lacking the first 18 amino acids is fully active for polyadenylation of oligoA, but defective for pre-mRNA polyadenylation, suggesting that interactions at the N-terminus are important for enzyme function in the processing complex. We have now identified proteins that interact specifically with this region. Cft1 and Pta1 are subunits of the cleavage/polyadenylation factor, in which Pap1 resides, and Nab6 and Sub1 are nucleic-acid binding proteins with known links to 3' end processing. Our results suggest a novel mechanism for controlling Pap1 activity, and possible models invoking these newly-discovered interactions are discussed.
摘要
在酵母中，断裂前 mRNA 的 3' 端添加 poly(A) 对于 mRNA 成熟是必不可少的，该过程由 Pap1 催化。我们之前已经表明，缺乏前 18 个氨基酸的非活性 Pap1 突变体完全能够对寡聚 A 进行多聚腺苷酸化，但对于前 mRNA 多聚腺苷酸化却是有缺陷的，这表明 N 端的相互作用对于酶在加工复合物中的功能很重要。我们现在已经鉴定出与该区域特异性相互作用的蛋白质。Cft1 和 Pta1 是切割/多聚腺苷酸化因子的亚基，Pap1 就位于其中，而 Nab6 和 Sub1 是具有已知与 3' 端加工相关的核酸结合蛋白。我们的结果表明了一种控制 Pap1 活性的新机制，并讨论了可能涉及这些新发现的相互作用的模型。
相似文献
1
Novel interactions at the essential N-terminus of poly(A) polymerase that could regulate poly(A) addition in Saccharomyces cerevisiae.聚腺苷酸化酶基本 N 端的新相互作用，可调节酿酒酵母中的聚腺苷酸化添加。
FEBS Lett. 2012 Apr 24;586(8):1173-8. doi: 10.1016/j.febslet.2012.03.036. Epub 2012 Mar 24.
2
Structure of yeast poly(A) polymerase in complex with a peptide from Fip1, an intrinsically disordered protein.与内在无序蛋白Fip1的一个肽段结合的酵母多聚腺苷酸聚合酶的结构。
Biochemistry. 2008 Jul 1;47(26):6859-69. doi: 10.1021/bi800204k. Epub 2008 Jun 7.
3
Efficient mRNA polyadenylation requires a ubiquitin-like domain, a zinc knuckle, and a RING finger domain, all contained in the Mpe1 protein.高效的mRNA聚腺苷酸化需要一个泛素样结构域、一个锌指结构和一个RING指结构域，所有这些结构域都包含在Mpe1蛋白中。
Mol Cell Biol. 2014 Nov;34(21):3955-67. doi: 10.1128/MCB.00077-14. Epub 2014 Aug 18.
4
A flexible linker region in Fip1 is needed for efficient mRNA polyadenylation.Fip1 中的柔性连接区对于高效的 mRNA 多聚腺苷酸化是必需的。
RNA. 2011 Apr;17(4):652-64. doi: 10.1261/rna.2273111. Epub 2011 Jan 31.
5
Evidence for a complex of transcription factor IIB with poly(A) polymerase and cleavage factor 1 subunits required for gene looping.基因环化所需的转录因子 IIB 与多聚（A）聚合酶和切割因子 1 亚基复合物的证据。
J Biol Chem. 2011 Sep 30;286(39):33709-18. doi: 10.1074/jbc.M110.193870. Epub 2011 Aug 11.
6
Pta1, a component of yeast CF II, is required for both cleavage and poly(A) addition of mRNA precursor.Pta1是酵母CF II的一个组成部分，它对于mRNA前体的切割和聚腺苷酸化都是必需的。
Mol Cell Biol. 1999 Nov;19(11):7733-40. doi: 10.1128/MCB.19.11.7733.
7
Activation of the Endonuclease that Defines mRNA 3' Ends Requires Incorporation into an 8-Subunit Core Cleavage and Polyadenylation Factor Complex.定义mRNA 3'末端的核酸内切酶的激活需要整合到一个由8个亚基组成的核心切割和聚腺苷酸化因子复合物中。
Mol Cell. 2019 Mar 21;73(6):1217-1231.e11. doi: 10.1016/j.molcel.2018.12.023. Epub 2019 Feb 5.
8
Fip1 regulates the activity of Poly(A) polymerase through multiple interactions.Fip1通过多种相互作用调节聚腺苷酸聚合酶的活性。
Mol Cell Biol. 2001 Mar;21(6):2026-37. doi: 10.1128/MCB.21.6.2026-2037.2001.
9
A multisubunit 3' end processing factor from yeast containing poly(A) polymerase and homologues of the subunits of mammalian cleavage and polyadenylation specificity factor.一种来自酵母的多亚基3'端加工因子，包含多聚腺苷酸聚合酶以及哺乳动物切割和聚腺苷酸化特异性因子亚基的同源物。
EMBO J. 1997 Aug 1;16(15):4727-37. doi: 10.1093/emboj/16.15.4727.
10
The FIP1 gene encodes a component of a yeast pre-mRNA polyadenylation factor that directly interacts with poly(A) polymerase.FIP1基因编码酵母前体mRNA聚腺苷酸化因子的一个组分，该组分可直接与聚腺苷酸聚合酶相互作用。
Cell. 1995 May 5;81(3):379-89. doi: 10.1016/0092-8674(95)90391-7.
引用本文的文献
1
Birth of a poly(A) tail: mechanisms and control of mRNA polyadenylation.mRNA 多聚腺苷酸化的产生机制与调控
FEBS Open Bio. 2023 Jul;13(7):1140-1153. doi: 10.1002/2211-5463.13528. Epub 2022 Dec 7.
2
Polyadenylation and nuclear export of mRNAs.mRNA 的多聚腺苷酸化和核输出。
J Biol Chem. 2019 Mar 1;294(9):2977-2987. doi: 10.1074/jbc.REV118.005594. Epub 2019 Jan 25.
3
Enhancement of protein production via the strong DIT1 terminator and two RNA-binding proteins in Saccharomyces cerevisiae.通过强 DIT1 终止子和两种 RNA 结合蛋白在酿酒酵母中增强蛋白质生产。
Sci Rep. 2016 Nov 15;6:36997. doi: 10.1038/srep36997.
4
Efficient mRNA polyadenylation requires a ubiquitin-like domain, a zinc knuckle, and a RING finger domain, all contained in the Mpe1 protein.高效的mRNA聚腺苷酸化需要一个泛素样结构域、一个锌指结构和一个RING指结构域，所有这些结构域都包含在Mpe1蛋白中。
Mol Cell Biol. 2014 Nov;34(21):3955-67. doi: 10.1128/MCB.00077-14. Epub 2014 Aug 18.
5
Delineating the structural blueprint of the pre-mRNA 3'-end processing machinery.描绘 pre-mRNA 3'-端加工机制的结构蓝图。
Mol Cell Biol. 2014 Jun;34(11):1894-910. doi: 10.1128/MCB.00084-14. Epub 2014 Mar 3.
6
Crystal structure of human poly(A) polymerase gamma reveals a conserved catalytic core for canonical poly(A) polymerases.人多聚腺苷酸聚合酶 γ 的晶体结构揭示了经典多聚腺苷酸聚合酶的保守催化核心。
J Mol Biol. 2014 Jan 9;426(1):43-50. doi: 10.1016/j.jmb.2013.09.025. Epub 2013 Sep 25.
7
Disengaging polymerase: terminating RNA polymerase II transcription in budding yeast.脱离聚合酶：在芽殖酵母中终止RNA聚合酶II转录
Biochim Biophys Acta. 2013 Jan;1829(1):174-85. doi: 10.1016/j.bbagrm.2012.10.003. Epub 2012 Oct 17.
本文引用的文献
1
Rrp6p controls mRNA poly(A) tail length and its decoration with poly(A) binding proteins.Rrp6p 控制 mRNA 多聚（A）尾的长度及其与多聚（A）结合蛋白的结合。
Mol Cell. 2012 Jul 27;47(2):267-80. doi: 10.1016/j.molcel.2012.05.005. Epub 2012 Jun 7.
2
Sub1 and RPA associate with RNA polymerase II at different stages of transcription.Sub1 和 RPA 在转录的不同阶段与 RNA 聚合酶 II 结合。
Mol Cell. 2011 Nov 4;44(3):397-409. doi: 10.1016/j.molcel.2011.09.013.
3
Control of poly(A) tail length.聚腺苷酸尾长度的控制。
Wiley Interdiscip Rev RNA. 2011 May-Jun;2(3):348-61. doi: 10.1002/wrna.56. Epub 2010 Nov 17.
4
Pre-mRNA 3'-end processing complex assembly and function.前体 mRNA 3'-端加工复合物的组装和功能。
Wiley Interdiscip Rev RNA. 2011 May-Jun;2(3):321-35. doi: 10.1002/wrna.54. Epub 2010 Oct 18.
5
Rrp6, Rrp47 and cofactors of the nuclear exosome.核小体 exosome 的 Rrp6、Rrp47 及其辅助因子。
Adv Exp Med Biol. 2010;702:91-104.
6
A flexible linker region in Fip1 is needed for efficient mRNA polyadenylation.Fip1 中的柔性连接区对于高效的 mRNA 多聚腺苷酸化是必需的。
RNA. 2011 Apr;17(4):652-64. doi: 10.1261/rna.2273111. Epub 2011 Jan 31.
7
To polyadenylate or to deadenylate: that is the question.多聚腺苷酸化还是去腺苷酸化：这是个问题。
Cell Cycle. 2010 Nov 15;9(22):4437-49. doi: 10.4161/cc.9.22.13887.
8
The genetic landscape of a cell.细胞的基因图谱。
Science. 2010 Jan 22;327(5964):425-31. doi: 10.1126/science.1180823.
9
A physical and functional link between splicing factors promotes pre-mRNA 3' end processing.剪接因子之间的物理和功能联系促进前体mRNA 3'末端加工。
Nucleic Acids Res. 2009 Aug;37(14):4672-83. doi: 10.1093/nar/gkp470. Epub 2009 Jun 8.
10
Sub1 functions in osmoregulation and in transcription by both RNA polymerases II and III.Sub1在渗透调节以及RNA聚合酶II和III的转录过程中发挥作用。
Mol Cell Biol. 2009 Apr;29(8):2308-21. doi: 10.1128/MCB.01841-08. Epub 2009 Feb 9.
Zotero 插件