Bonnet Romaric, Murat Pierre, Spinelli Nicolas, Defrancq Eric
Département de Chimie Moléculaire UMR CNRS 5250, Université Joseph Fourier BP 53, 38041 Grenoble cedex 9, France.
Chem Commun (Camb). 2012 Jun 18;48(48):5992-4. doi: 10.1039/c2cc32010j. Epub 2012 May 10.
The use of a peptidic template and orthogonal oxime ligation and CuAAC reaction allows a stepwise approach for the design of antiparallel tetrameric DNA structure. As a proof of concept template assisted synthesis of i-motif DNA was achieved. As anticipated, a dramatic improvement in the stability of such a tetrameric structure was observed. More interestingly the formation of the i-motif was still observed at neutral pH, which could be attributed to the spatial proximity of the basic centers.
使用肽模板以及正交肟连接和铜催化的叠氮化物-炔烃环加成反应（CuAAC反应），可以采用逐步方法来设计反平行四聚体DNA结构。作为概念验证，实现了模板辅助的i-基序DNA合成。正如预期的那样，观察到这种四聚体结构的稳定性有了显著提高。更有趣的是，在中性pH条件下仍观察到i-基序的形成，这可能归因于碱性中心在空间上的接近。
