Department of Pharmaceutical Sciences, University of Bologna, Italy.
Anaerobe. 2012 Aug;18(4):386-91. doi: 10.1016/j.anaerobe.2012.04.014. Epub 2012 May 8.
In the current study, batch culture fermentations on fecal samples of 3 healthy individuals were performed to assess the effect of the addition of prebiotics (FOS), probiotics (Bifidobacterium longum Bar33 and Lactobacillus helveticus Bar13) and synbiotics (B. longum Bar33 + L. helveticus Bar13 + FOS) on the fecal metabolic profiles. A total of 84 different metabolites belonging to the families of sulfur compounds, nitrogen compounds, aldehydes, ketones, esters, alcohols, phenols, organic acids, and hydrocarbons were detected by GC-MS/SPME analysis. The highest number of metabolites varied in concentration in the models with added FOS and synbiotics, where several metabolic signatures were found in common. The increase of butyrate represented the greatest variation registered after the addition of FOS alone. Following the B. longum Bar33 addition, 2-methyl butyrate underwent the most evident variation. In the batch fermentation with added L. helveticus Bar13, the decrease of pyridine and butandiene was observed together with the increase of 2-methyl-5-ethyl-pyrazine, 2-butanone and butyrate. The modification of the fecal metabolic profiles induced by the simultaneous addition of B. longum Bar33 and L. helveticus Bar13 was very similar to that observed after the supplementation with L. helveticus Bar13, regarding mainly the decrease of pyridine and the increase of butyrate.
在本研究中，对 3 名健康个体的粪便样本进行了分批培养发酵，以评估添加益生元（FOS）、益生菌（长双歧杆菌 Bar33 和瑞士乳杆菌 Bar13）和合生元（长双歧杆菌 Bar33+瑞士乳杆菌 Bar13+FOS）对粪便代谢谱的影响。通过 GC-MS/SPME 分析共检测到 84 种不同的代谢物，属于硫化合物、氮化合物、醛类、酮类、酯类、醇类、酚类、有机酸和碳氢化合物家族。在添加 FOS 和合生元的模型中，浓度变化的代谢物数量最多，其中发现了几种共同的代谢特征。单独添加 FOS 后，丁酸的增加代表了登记的最大变化。在添加长双歧杆菌 Bar33 的分批发酵中，2-甲基丁酸经历了最明显的变化。在添加瑞士乳杆菌 Bar13 的分批发酵中，观察到吡啶和丁二烯的减少，同时 2-甲基-5-乙基-吡嗪、2-丁酮和丁酸的增加。长双歧杆菌 Bar33 和瑞士乳杆菌 Bar13 同时添加引起的粪便代谢谱的改变与瑞士乳杆菌 Bar13 补充后的改变非常相似，主要是关于吡啶的减少和丁酸的增加。
