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强力且选择性结合阿米洛利到 RNA 双链体中的无碱基位点：热力学特性分析和 microRNA 检测。

Strong and selective binding of amiloride to an abasic site in RNA duplexes: thermodynamic characterization and microRNA detection.

机构信息

Department of Chemistry, Graduate School of Science, Tohoku University, Aoba-ku, Sendai 980-8578, Japan.

出版信息

Angew Chem Int Ed Engl. 2012 Jun 25;51(26):6369-72. doi: 10.1002/anie.201201790. Epub 2012 May 15.

DOI:10.1002/anie.201201790
PMID:22589079
Abstract

Firmly tied: The binding affinity of amiloride for an abasic (AP) site-containing RNA duplex is two orders of magnitude superior to the affinity of the corresponding AP site-containing DNA duplex. The observed high binding affinity for the RNA duplex arises from a favorable enthalpy gain. The binding-induced fluorescence response of amiloride is applicable to microRNA detection.

摘要

牢固结合

阿米洛利与含 AP 位点的 RNA 双链体的结合亲和力比相应的含 AP 位点的 DNA 双链体的亲和力高出两个数量级。对 RNA 双链体的高结合亲和力源于有利的焓变收益。阿米洛利的结合诱导荧光响应可适用于 microRNA 检测。

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