Department of Bioscience Research, College of Dentistry, The University of Tennessee Health Science Center, Memphis, TN 38163, USA.
Electrophoresis. 2012 Apr;33(8):1311-21. doi: 10.1002/elps.201100505.
The article describes an innovative delivery system based on the principles of dielectrophoresis to transport drugs directly into site-specific intraoral targets. The hypothesis that a drug can be driven into tooth enamel during the application of an applied electrical potential difference was tested by the authors in in vitro studies comparing dielectrophoresis to diffusion to transport carbamide peroxide and fluoride. The studies showed that these agents can be transported directly into teeth using an alternating current (AC) electric field more effectively than diffusion. It was found that a 20-min bleaching treatment on human teeth with dielectrophoresis increased carbamide peroxide absorption by 104% and, on average, improved the change in shade guide unit 14 times from 0.6 SGU to 9 SGU. After applying a 1.23% acidulated phosphate fluoride gel to bovine incisors for 20 min by dielectrophoresis or diffusion, analysis with wavelength dispersive spectrometry determined that dielectrophoresis doubled fluoride uptake in the superficial layers compared to diffusion, and drove the fluoride significantly deeper into enamel with an uptake 600% higher than diffusion at 50 μm depth. Finally, dielectrophoresis promises to be a viable model that can potentially be used clinically to deliver other targeted drugs of variable molecular weight and structure.
本文描述了一种基于电介质电泳原理的创新递药系统，可将药物直接输送到特定的口腔内靶标部位。作者通过体外研究测试了药物在施加外加电势差时可进入牙釉质的假设，这些研究比较了电介质电泳和扩散对输送过氧脲和氟化物的作用。研究表明，与扩散相比，使用交流电（AC）电场可更有效地将这些药物直接输送到牙齿中。研究发现，在人类牙齿上进行 20 分钟的电介质电泳漂白处理，可使过氧脲的吸收增加 104%，平均而言，可将色标单位的变化从 0.6 SGU 提高 14 倍至 9 SGU。通过电介质电泳或扩散将 1.23%的酸性磷酸氟凝胶施用到牛切牙 20 分钟后，用波长色散光谱法分析发现，与扩散相比，电介质电泳使表面层的氟摄取量增加了一倍，并且使氟化物显著更深地进入牙釉质，在 50μm 深度处的摄取量比扩散高 600%。最后，电介质电泳有望成为一种可行的模型，可潜在地用于临床输送其他具有不同分子量和结构的靶向药物。
